Projects per year
Abstract
Organic light emitting diodes (OLEDs) are in widespread use in today’s mobile phones and are likely to drive the next generation of large area displays and solid-state lighting. Here we show steps towards their utility as a platform technology for biophotonics, by demonstrating devices capable of optical controlling behaviour in live animals. Using devices with pin OLED architecture, sufficient illumination intensity (0.3 mW.mm-2) to activate channelrhodopsins (ChRs) in vivo was reliably achieved at low operating voltages (5 V). In Drosophila melanogaster third instar larvae expressing ChR2(H134R) in motor neurons, we found that pulsed illumination from blue and green OLEDs triggered robust and reversible contractions in animals. This response was temporally coupled to the timing of OLED illumination. With blue OLED illumination, the initial rate and overall size of the behavioural response was strongest. Green OLEDs achieved roughly 70% of the response observed with blue OLEDs. Orange OLEDs did not produce contractions in larvae, in agreement with the spectral response of ChR2(H134R). The device configuration presented here could be modified to accommodate other small model organisms, cell cultures or tissue slices and the ability of OLEDs to provide patterned illumination and spectral tuning can broaden their utility in optogenetics experiments further.
Original language | English |
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Article number | 31117 |
Number of pages | 8 |
Journal | Scientific Reports |
Volume | 6 |
DOIs | |
Publication status | Published - 3 Aug 2016 |
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Dive into the research topics of 'High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour'. Together they form a unique fingerprint.Projects
- 3 Finished
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ISSF3 Wellcome Trust Award: Institutional Strategic Support Fund (ISSF3)
Brown, C. T. A. (PI)
1/03/17 → 29/02/20
Project: Standard
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H2020 MSCA Fellowship NeurOLED: H2020 MSCA Fellowship (Caroline Murawski)
Gather, M. C. (PI)
1/03/16 → 19/07/18
Project: Fellowship
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Neurons feel the force: Neurons feel the force-New photonic tools to unravel the development of the nervous system
Gather, M. C. (PI)
1/10/13 → 31/05/17
Project: Standard
Datasets
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Data underpinning - High-brightness organic light-emitting diodes for optogenetic control of Drosophila locomotor behaviour
Morton, A. (Creator), Murawski, C. (Creator), Pulver, S. (Creator) & Gather, M. C. (Creator), University of St Andrews, 3 Aug 2016
DOI: 10.17630/75928445-e77c-4f79-9b7f-d7d697ab7f29
Dataset
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