Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: The polymerase (P3Dpol) as an immunodominant immunogen

T Collen; J Baron; A Childerstone; A Corteyn; TR Doel; M Flint; M Garcia-Valcarcel; RME Parkhouse; Martin Denis Ryan

Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: The polymerase (P3Dpol) as an immunodominant immunogen

T Collen, J Baron, A Childerstone, A Corteyn, TR Doel, M Flint, M Garcia-Valcarcel, RME Parkhouse, Martin Denis Ryan

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3D(pol) by PBMC and CD4(+) T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VPI was recognised in an homotypic context whereas VP2, VP3, VP4 and P3D(pol) were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3D(pol) was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3D(pol) ii: purified virus. Thus, P3D(pol) is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine. (C) 1998 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	125-133
Number of pages	9
Journal	Virus Research
Volume	56
Publication status	Published - Aug 1998

Keywords

foot-and-mouth disease virus
T-cell recognition
serotype-cross-reactivity
RNA-dependent RNA polymerase
MOUTH-DISEASE VIRUS
SYNTHETIC PEPTIDE
B-CELL
ANTIBODY-RESPONSE
FOOT
CATTLE
PROTECTION
VACCINES
HEMAGGLUTININ
DETERMINANTS

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@article{13e7cc544ab24d6fa4fc19346045f99d,

title = "Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: The polymerase (P3Dpol) as an immunodominant immunogen",

abstract = "In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3D(pol) by PBMC and CD4(+) T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VPI was recognised in an homotypic context whereas VP2, VP3, VP4 and P3D(pol) were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3D(pol) was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3D(pol) ii: purified virus. Thus, P3D(pol) is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine. (C) 1998 Elsevier Science B.V. All rights reserved.",

keywords = "foot-and-mouth disease virus, T-cell recognition, serotype-cross-reactivity, RNA-dependent RNA polymerase, MOUTH-DISEASE VIRUS, SYNTHETIC PEPTIDE, B-CELL, ANTIBODY-RESPONSE, FOOT, CATTLE, PROTECTION, VACCINES, HEMAGGLUTININ, DETERMINANTS",

author = "T Collen and J Baron and A Childerstone and A Corteyn and TR Doel and M Flint and M Garcia-Valcarcel and RME Parkhouse and Ryan, {Martin Denis}",

year = "1998",

month = aug,

language = "English",

volume = "56",

pages = "125--133",

journal = "Virus Research",

issn = "0168-1702",

publisher = "Elsevier",

}

TY - JOUR

T1 - Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: The polymerase (P3Dpol) as an immunodominant immunogen

AU - Collen, T

AU - Baron, J

AU - Childerstone, A

AU - Corteyn, A

AU - Doel, TR

AU - Flint, M

AU - Garcia-Valcarcel, M

AU - Parkhouse, RME

AU - Ryan, Martin Denis

PY - 1998/8

Y1 - 1998/8

N2 - In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3D(pol) by PBMC and CD4(+) T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VPI was recognised in an homotypic context whereas VP2, VP3, VP4 and P3D(pol) were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3D(pol) was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3D(pol) ii: purified virus. Thus, P3D(pol) is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine. (C) 1998 Elsevier Science B.V. All rights reserved.

AB - In this study we have examined the recognition of VP0, VP1, VP2, VP3 and P3D(pol) by PBMC and CD4(+) T-cells from infected, vaccinated-challenged, and multiply-vaccinated (O1, A24, C1 or ASIA1) cattle using recombinant proteins of an O1 serotype virus. The structural protein VPI was recognised in an homotypic context whereas VP2, VP3, VP4 and P3D(pol) were also recognised by T-cells from animals exposed to heterotypic viruses. Only the non-structural protein P3D(pol) was consistently recognised by T-cells from the majority of animals examined and heterotypic recognition correlated with the presence of serologically detectable P3D(pol) ii: purified virus. Thus, P3D(pol) is a major cross-reactive immunodeterminant of FMDV, eliciting heterotypic T-cell responses and, therefore, with possible potential for inclusion in a subunit vaccine. (C) 1998 Elsevier Science B.V. All rights reserved.

KW - foot-and-mouth disease virus

KW - T-cell recognition

KW - serotype-cross-reactivity

KW - RNA-dependent RNA polymerase

KW - MOUTH-DISEASE VIRUS

KW - SYNTHETIC PEPTIDE

KW - B-CELL

KW - ANTIBODY-RESPONSE

KW - FOOT

KW - CATTLE

KW - PROTECTION

KW - VACCINES

KW - HEMAGGLUTININ

KW - DETERMINANTS

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M3 - Article

SN - 0168-1702

VL - 56

SP - 125

EP - 133

JO - Virus Research

JF - Virus Research

ER -

Heterotypic recognition of recombinant FMDV proteins by bovine T-cells: The polymerase (P3Dpol) as an immunodominant immunogen

Abstract

Keywords

UN SDGs

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