Haem-iron plays a key role in the regulation of the Ess/type VII secretion system of Staphylococcus aureus RN6390

M Guillermina Casabona, Holger Kneuper, Daniela Alferes de Lima, Catriona P Harkins, Martin Zoltner, Erik Hjerde, Matthew T. G. Holden, Tracy Palmer

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
2 Downloads (Pure)

Abstract

The Staphylococcus aureus type VII protein secretion system (T7SS) plays important roles in virulence and intra-species competition. Here we show that the T7SS in strain RN6390 is activated by supplementing the growth medium with haemoglobin, and its cofactor haemin (haem B). Transcript analysis and secretion assays suggest that activation by haemin occurs at a transcriptional and a post-translational level. Loss of T7 secretion activity by deletion of essC results in upregulation of genes required for iron acquisition. Taken together these findings suggest that the T7SS plays a role in iron homeostasis in at least some S. aureus strains.

Original languageEnglish
Pages (from-to)1839-1850
JournalMicrobiology
Volume163
Issue number12
Early online date24 Nov 2017
DOIs
Publication statusPublished - 1 Dec 2017

Keywords

  • Staphylococcus aureus
  • Protein secretion
  • Iron homeostasis
  • RNA-sequencing

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