Genomic, evolutionary, and expression analyses of cee, an ancient gene involved in normal growth and development.

Jorge Fernandes, D J Macqueen, Hung-Tai Lee, Ian Alistair Johnston

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The cee (conserved edge expressed protein) gene was recently identified in a genome-wide screen to discover genes associated with myotube formation in fast muscle of pufferfish. Comparative genomic analyses indicate that cee arose some 1.6 - 1.8 billion years ago and is found as a single-copy gene in most eukaryotic genomes examined. The complexity of its structure varies from an intronless gene in yeast and tunicates to nine exons and eight introns in vertebrates. cee is particularly conserved among vertebrates and is located in a syntenic region within tetrapods and between teleosts and invertebrates. Low dN/dS ratios in the cee coding region (0.02 - 0.09) indicate that the Cee protein is under strong purifying selection. In Atlantic salmon, cee is expressed in the superficial layers of developing organs and tissues. These data, together with functional screens in yeast and Caenorhabditis elegans, indicate that cee has a hitherto uncharacterized role in normal growth and development. (c) 2008 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)315-325
Number of pages11
JournalGenomics
Volume91
Issue number4
Early online date31 Jan 2008
DOIs
Publication statusPublished - Apr 2008

Keywords

  • cee
  • conserved edge-expressed protein
  • myogenesis
  • development
  • purifying selection
  • MUSCLE-FIBER NUMBER
  • SACCHAROMYCES-CEREVISIAE
  • PHYLOGENETIC INFERENCE
  • ATLANTIC SALMON
  • FISH
  • PROTEIN
  • ZEBRAFISH
  • YEAST
  • VERTEBRATES
  • MODELS

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