Genome-wide association study of white blood cell counts in patients with ischemic stroke

Nuria P Torres-Aguila, Caty Carrera, Anne-Katrine Giese, Natalia Cullell, Elena Muiño, Jara Cárcel-Márquez, Cristina Gallego-Fabrega, Jonathan González-Sánchez, María Del Mar Freijo, José Álvarez-Sabín, Carlos Molina, Marc Ribó, Jordi Jimenez-Conde, Jaume Roquer, Tomás Sobrino, Francisco Campos, José Castillo, Lucia Muñoz-Narbona, Elena Lopez-Cancio, Antoni DàvalosRosa Diaz-Navarro, Silvia Tur, Cristòfol Vives-Bauza, Gemma Serrano-Heras, Tomás Segura, Jerzy Krupinski, Raquel Delgado-Mederos, Joan Martí-Fàbregas, Laura Heitsch, Laura Ibañez, Carlos Cruchaga, Natalia S Rost, Joan Montaner, Jin-Moo Lee, Israel Fernandez-Cadenas

Research output: Contribution to journalArticlepeer-review

Abstract

Background and Purpose- Immune cells play a key role in the first 24h poststroke (acute phase), being associated with stroke outcome. We aimed to find genetic risk factors associated with leukocyte counts during the acute phase of stroke. Methods- Ischemic stroke patients with leukocyte counts data during the first 24h were included. Genome-wide association study and gene expression studies were performed. Results- Our genome-wide association study, which included 2064 (Discovery) and 407 (Replication) patients, revealed a new locus (14q24.3) associated with leukocyte counts. After Joint analysis (n=2471) 5 more polymorphisms reached genome-wide significance (P<5×10-8). The 14q24.3 locus was associated with acute stroke outcome (rs112809786, P=0.036) and with ACOT1 and PTGR2 gene expression. Previous polymorphisms associated with leukocyte counts in general-population did not show any significance in our study. Conclusions- We have found the first locus associated with leukocyte counts in ischemic stroke, also associated with acute outcome. Genetic analysis of acute endophenotypes could be useful to find the genetic factors associated with stroke outcome. Our findings suggested a different modulation of immune cells in stroke compared with healthy conditions.
Original languageEnglish
Pages (from-to)3618-3621
Number of pages4
JournalStroke
Volume50
Issue number12
DOIs
Publication statusPublished - Dec 2019

Keywords

  • Aged
  • Aged, 80 and over
  • Brain Ischemia/genetics
  • Chromosomes, Human, Pair 14/genetics
  • Female
  • Genome-Wide Association Study
  • Humans
  • Leukocyte Count
  • Leukocytes/immunology
  • Male
  • Middle Aged
  • Polymorphism, Single Nucleotide
  • Prognosis
  • Stroke/genetics

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