Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus

EI Campbell; Sheila Eileen Unkles; James Robertson Kinghorn; C Panter

doi:10.1080/09583150220128176

Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus

EI Campbell
, Sheila Eileen Unkles
, James Robertson Kinghorn
, C Panter

Research output: Contribution to journal › Article › peer-review

Abstract

The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.

Original language	English
Pages (from-to)	395-399
Number of pages	5
Journal	Biocontrol Science and Technology
Volume	12
DOIs	https://doi.org/10.1080/09583150220128176
Publication status	Published - Jun 2002

Keywords

fungal pathogen of mosquitoes
Culicinomyces clavisporus
genetic transformation
antibiotic resistance
ASPERGILLUS

Access to Document

10.1080/09583150220128176

Handle.net

Persistent link to portal record

Cite this

@article{01eab39e1be74f7f9dcc206e7c77dee3,

title = "Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus",

abstract = "The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.",

keywords = "fungal pathogen of mosquitoes, Culicinomyces clavisporus, genetic transformation, antibiotic resistance, ASPERGILLUS",

author = "EI Campbell and Unkles, \{Sheila Eileen\} and Kinghorn, \{James Robertson\} and C Panter",

note = "Collaboration with C Panter - bulk of experimental work carried out in my lab at St Andrews",

year = "2002",

month = jun,

doi = "10.1080/09583150220128176",

language = "English",

volume = "12",

pages = "395--399",

journal = "Biocontrol Science and Technology",

issn = "0958-3157",

publisher = "Taylor and Francis",

}

TY - JOUR

T1 - Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus

AU - Campbell, EI

AU - Unkles, Sheila Eileen

AU - Kinghorn, James Robertson

AU - Panter, C

N1 - Collaboration with C Panter - bulk of experimental work carried out in my lab at St Andrews

PY - 2002/6

Y1 - 2002/6

N2 - The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.

AB - The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.

KW - fungal pathogen of mosquitoes

KW - Culicinomyces clavisporus

KW - genetic transformation

KW - antibiotic resistance

KW - ASPERGILLUS

UR - https://www.scopus.com/pages/publications/0036105164

U2 - 10.1080/09583150220128176

DO - 10.1080/09583150220128176

M3 - Article

SN - 0958-3157

VL - 12

SP - 395

EP - 399

JO - Biocontrol Science and Technology

JF - Biocontrol Science and Technology

ER -

Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus

Abstract

Keywords

Access to Document

Handle.net

Other files and links

Fingerprint

Cite this