TY - JOUR
T1 - Genetic transformation of the mosquito pathogenic fungus Culicinomyces clavisporus
AU - Campbell, EI
AU - Unkles, Sheila Eileen
AU - Kinghorn, James Robertson
AU - Panter, C
N1 - Collaboration with C Panter - bulk of experimental work carried out in my lab at St Andrews
PY - 2002/6
Y1 - 2002/6
N2 - The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.
AB - The mosquito fungal pathogen Culicinomyces clavisporus was found to be sensitive to the antibiotics hygromycin B and phleomycin but resistant to kanamycin and geneticin. Protoplasts were genetically transformed to give strains resistant to phleomycin and hygromycin B. The results show that multiple integrations of transforming DNA can occur and that heterologous fungal gene expression signals are functional in C. clavisporus.
KW - fungal pathogen of mosquitoes
KW - Culicinomyces clavisporus
KW - genetic transformation
KW - antibiotic resistance
KW - ASPERGILLUS
UR - http://www.scopus.com/inward/record.url?scp=0036105164&partnerID=8YFLogxK
U2 - 10.1080/09583150220128176
DO - 10.1080/09583150220128176
M3 - Article
SN - 0958-3157
VL - 12
SP - 395
EP - 399
JO - Biocontrol Science and Technology
JF - Biocontrol Science and Technology
ER -