Genetic fusion of proteins to the SIV Tat protein enhances their immunogenicity.

Y-H Chen; A Diassiti; Richard Edward Randall

doi:10.1016/j.vaccine.2005.09.012

Genetic fusion of proteins to the SIV Tat protein enhances their immunogenicity.

Y-H Chen, A Diassiti, Richard Edward Randall

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The potential of genetically fusing recombinant proteins to the simian immunodeficiency virus (SIV) Tat protein has been investigated. The recombinant SIV Tat protein was initially expressed in very low amounts in E. coli, but optimization of the coding sequence for translation in the bacterial host significantly improved protein expression. Whilst fusion of SIV Tat to an experimental antigen (GST) facilitated the binding of the antigen to cell surfaces it did not appear to facilitate the transport of the protein into the cytosol. The immunogenicity of GST was significantly enhanced, in the absence of adjuvants, when fused to SIV Tat, with the induction of IgG1 and IgG2a antibodies indicative of a Th 1 response being induced. However, no evidence was obtained that such an immunization scheme efficiently induced a CTL response. (c) 2005 Elsevier Ltd. All rights reserved.

Original language	English
Pages (from-to)	708-715
Number of pages	8
Journal	Vaccine
Volume	24
DOIs	https://doi.org/10.1016/j.vaccine.2005.09.012
Publication status	Published - 6 Feb 2006

Keywords

SIV Tat fusion proteins
Th1/Th2
IgG2a
adjuvant
immunogenicity
HUMAN-IMMUNODEFICIENCY-VIRUS
MHC CLASS-I
T-CELL
TRANSDUCTION DOMAIN
ESCHERICHIA-COLI
SOLUBLE-PROTEIN
DELIVERY
VACCINE
RESPONSES
ANTIGEN

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2005.09.012

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title = "Genetic fusion of proteins to the SIV Tat protein enhances their immunogenicity.",

abstract = "The potential of genetically fusing recombinant proteins to the simian immunodeficiency virus (SIV) Tat protein has been investigated. The recombinant SIV Tat protein was initially expressed in very low amounts in E. coli, but optimization of the coding sequence for translation in the bacterial host significantly improved protein expression. Whilst fusion of SIV Tat to an experimental antigen (GST) facilitated the binding of the antigen to cell surfaces it did not appear to facilitate the transport of the protein into the cytosol. The immunogenicity of GST was significantly enhanced, in the absence of adjuvants, when fused to SIV Tat, with the induction of IgG1 and IgG2a antibodies indicative of a Th 1 response being induced. However, no evidence was obtained that such an immunization scheme efficiently induced a CTL response. (c) 2005 Elsevier Ltd. All rights reserved.",

keywords = "SIV Tat fusion proteins, Th1/Th2, IgG2a, adjuvant, immunogenicity, HUMAN-IMMUNODEFICIENCY-VIRUS, MHC CLASS-I, T-CELL, TRANSDUCTION DOMAIN, ESCHERICHIA-COLI, SOLUBLE-PROTEIN, DELIVERY, VACCINE, RESPONSES, ANTIGEN",

author = "Y-H Chen and A Diassiti and Randall, {Richard Edward}",

year = "2006",

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day = "6",

doi = "10.1016/j.vaccine.2005.09.012",

language = "English",

volume = "24",

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journal = "Vaccine",

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}

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T1 - Genetic fusion of proteins to the SIV Tat protein enhances their immunogenicity.

AU - Chen, Y-H

AU - Diassiti, A

AU - Randall, Richard Edward

PY - 2006/2/6

Y1 - 2006/2/6

N2 - The potential of genetically fusing recombinant proteins to the simian immunodeficiency virus (SIV) Tat protein has been investigated. The recombinant SIV Tat protein was initially expressed in very low amounts in E. coli, but optimization of the coding sequence for translation in the bacterial host significantly improved protein expression. Whilst fusion of SIV Tat to an experimental antigen (GST) facilitated the binding of the antigen to cell surfaces it did not appear to facilitate the transport of the protein into the cytosol. The immunogenicity of GST was significantly enhanced, in the absence of adjuvants, when fused to SIV Tat, with the induction of IgG1 and IgG2a antibodies indicative of a Th 1 response being induced. However, no evidence was obtained that such an immunization scheme efficiently induced a CTL response. (c) 2005 Elsevier Ltd. All rights reserved.

AB - The potential of genetically fusing recombinant proteins to the simian immunodeficiency virus (SIV) Tat protein has been investigated. The recombinant SIV Tat protein was initially expressed in very low amounts in E. coli, but optimization of the coding sequence for translation in the bacterial host significantly improved protein expression. Whilst fusion of SIV Tat to an experimental antigen (GST) facilitated the binding of the antigen to cell surfaces it did not appear to facilitate the transport of the protein into the cytosol. The immunogenicity of GST was significantly enhanced, in the absence of adjuvants, when fused to SIV Tat, with the induction of IgG1 and IgG2a antibodies indicative of a Th 1 response being induced. However, no evidence was obtained that such an immunization scheme efficiently induced a CTL response. (c) 2005 Elsevier Ltd. All rights reserved.

KW - SIV Tat fusion proteins

KW - Th1/Th2

KW - IgG2a

KW - adjuvant

KW - immunogenicity

KW - HUMAN-IMMUNODEFICIENCY-VIRUS

KW - MHC CLASS-I

KW - T-CELL

KW - TRANSDUCTION DOMAIN

KW - ESCHERICHIA-COLI

KW - SOLUBLE-PROTEIN

KW - DELIVERY

KW - VACCINE

KW - RESPONSES

KW - ANTIGEN

UR - http://www.scopus.com/inward/record.url?scp=31944438018&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2005.09.012

DO - 10.1016/j.vaccine.2005.09.012

M3 - Article

SN - 0264-410X

VL - 24

SP - 708

EP - 715

JO - Vaccine

JF - Vaccine

ER -

Genetic fusion of proteins to the SIV Tat protein enhances their immunogenicity.

Abstract

Keywords

UN SDGs

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