Gene Expression Enabling Synthetic Diversification of Natural Products: Chemogenetic Generation of Pacidamycin Analogs

Abhijeet Deb Roy, Sabine Grueschow, Nickiwe Cairns, Rebecca J. M. Goss*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

110 Citations (Scopus)

Abstract

Introduction of prnA, the halogenase gene from pyrrolnitrin biosynthesis, into Streptomyces coeruleorubidus resulted in efficient in situ chlorination of the uridyl peptide antibotic pacidamycin. The installed chlorine provided a selectably functionalizable handle enabling synthetic modification of the natural product using mild cross-coupling conditions in crude aqueous extracts of the culture broth.

Original languageEnglish
Pages (from-to)12243-12245
Number of pages3
JournalJournal of the American Chemical Society
Volume132
Issue number35
DOIs
Publication statusPublished - 8 Sept 2010

Keywords

  • PRECURSOR-DIRECTED BIOSYNTHESIS
  • STREPTOMYCES
  • MECHANISM
  • HALOGENASE
  • ANTIBIOTICS
  • CRYSTAL-STRUCTURE

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