Gene C2 of the monopartite geminivirus Tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus

R  van Wezel; Huanting Liu; P  Tien; J  Stanley; Y G  Hong

doi:10.1094/MPMI.2001.14.9.1125

Gene C2 of the monopartite geminivirus Tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus

R van Wezel, Huanting Liu, P Tien, J Stanley, Y G Hong

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.

Original language	English
Pages (from-to)	1125-1128
Number of pages	4
Journal	Molecular Plant-Microbe Interactions
Volume	14
Issue number	9
DOIs	https://doi.org/10.1094/MPMI.2001.14.9.1125
Publication status	Published - Sept 2001

Keywords

Begomovirus
Geminiviridae
putative transcriptional activator protein
WHITEFLY-TRANSMITTED GEMINIVIRUS
COAT PROTEIN PROMOTER
GENOME ORGANIZATION
EXPRESSION
DNA
TRANSACTIVATION
AC2
SUPPRESSION
ACTIVATION
SEQUENCE

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10.1094/MPMI.2001.14.9.1125

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title = "Gene C2 of the monopartite geminivirus Tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus",

abstract = "Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.",

keywords = "Begomovirus, Geminiviridae, putative transcriptional activator protein, WHITEFLY-TRANSMITTED GEMINIVIRUS, COAT PROTEIN PROMOTER, GENOME ORGANIZATION, EXPRESSION, DNA, TRANSACTIVATION, AC2, SUPPRESSION, ACTIVATION, SEQUENCE",

author = "{van Wezel}, R and Huanting Liu and P Tien and J Stanley and Hong, {Y G}",

year = "2001",

month = sep,

doi = "10.1094/MPMI.2001.14.9.1125",

language = "English",

volume = "14",

pages = "1125--1128",

journal = "Molecular Plant-Microbe Interactions",

issn = "0894-0282",

publisher = "American Phytopathological Society",

number = "9",

}

TY - JOUR

T1 - Gene C2 of the monopartite geminivirus Tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus

AU - van Wezel, R

AU - Liu, Huanting

AU - Tien, P

AU - Stanley, J

AU - Hong, Y G

PY - 2001/9

Y1 - 2001/9

N2 - Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.

AB - Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.

KW - Begomovirus

KW - Geminiviridae

KW - putative transcriptional activator protein

KW - WHITEFLY-TRANSMITTED GEMINIVIRUS

KW - COAT PROTEIN PROMOTER

KW - GENOME ORGANIZATION

KW - EXPRESSION

KW - DNA

KW - TRANSACTIVATION

KW - AC2

KW - SUPPRESSION

KW - ACTIVATION

KW - SEQUENCE

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UR - http://dx.doi.org/10.1094/MPMI.2001.14.9.1125

U2 - 10.1094/MPMI.2001.14.9.1125

DO - 10.1094/MPMI.2001.14.9.1125

M3 - Article

SN - 0894-0282

VL - 14

SP - 1125

EP - 1128

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

IS - 9

ER -

Gene C2 of the monopartite geminivirus Tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus

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Keywords

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