Abstract
The proteolytic profile after mild controlled trypsin cleavage of shark rectal gland Na,K-ATPase was characterized and compared to that of pig kidney Na,K-ATPase, and conditions for achieving N-terminal cleavage of the alpha-subunit at the T-2 trypsin cleavage site were established. Using such conditions, the shark enzyme N-terminus was much more susceptible to proteolysis than the pig enzyme. Nevertheless, the maximum hydrolytic activity was almost unaffected for the shark enzyme, whereas it was significantly decreased for the pig kidney enzyme. The apparent ATP affinity was unchanged for shark but increased for pig enzyme after N-terminal truncation. The main common effect following N-terminal truncation of shark and pig Na,K-ATPase is a shift in the E-1-E-2 conformational equilibrium toward El. The phosphorylation and the main rate-limiting E-2 - E-1 step are both accelerated after N-terminal truncation of the shark enzyme, but decreased significantly in the pig kidney enzyme. Some of the kinetic differences, like the acceleration of the phosphorylation reaction, following N-terminal truncation of the two preparations may be due to the fact that under the conditions used for N-terminal truncation, the C-terminal domain of the FXYD regulatory protein of the shark enzyme, PLMS or FXYD10, was also cleaved, whereas the gamma or FXYD2 of the pig enzyme was not. In the shark enzyme, N-terminal truncation of the a-subunit abolished association of exogenous PLMS with the a-subunit and the functional interactions were abrogated. Moreover, PKC phosphorylation of the preparation, which relieves PLMS inhibition of Na,K-ATPase activity, exposed the N-terminal trypsin cleavage site. It is suggested that PLMS interacts functionally with the N-terminus of the shark Na,K-ATPase to control the E-1-E-2 conformational transition of the enzyme and that such interactions may be controlled by regulatory protein kinase phosphorylation of the N-terminus. Such interactions are likely in shark enzyme where PLMS has been demonstrated by cross-linking to associate with the Na,K-ATPase A-domain.
Original language | English |
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Pages (from-to) | 13051-13062 |
Number of pages | 12 |
Journal | Biochemistry |
Volume | 44 |
Issue number | 39 |
DOIs | |
Publication status | Published - 4 Oct 2005 |
Keywords
- PHOSPHOLEMMAN-LIKE PROTEIN
- NA+,K+-ATPASE PUMP CYCLE
- EEL ANGUILLA-ANGUILLA
- ALPHA-SUBUNIT
- GAMMA-SUBUNIT
- KINASE-C
- CATALYTIC SUBUNIT
- STEADY-STATE
- CONFORMATIONAL-CHANGES
- TRYPTIC DIGESTION