Functional characterization of nematode effectors in plants

Axel A. Elling; John T. Jones

doi:10.1007/978-1-62703-986-4_9

Functional characterization of nematode effectors in plants

Research output: Contribution to journal › Article › peer-review

Abstract

Secreted effectors represent the molecular interface between the nematode and its host plant. Studies that aimed at deciphering molecular plant–nematode interactions are hampered by technical hurdles that prevent the generation of transgenic nematodes. However, RNA interference (RNAi) has proven to be a valuable tool to specifically knock-down nematode effector genes, both ex planta and in planta. Plantmediated RNAi of nematode genes not only facilitates functional characterization of effectors but also lends itself to a novel control strategy against plant-parasitic nematodes. Here, we describe currently used methods to silence genes in plant-parasitic cyst and root-knot nematodes.

Original language	English
Pages (from-to)	113-124
Number of pages	12
Journal	Methods in Molecular Biology
Volume	1127
DOIs	https://doi.org/10.1007/978-1-62703-986-4_9
Publication status	Published - 2014

Keywords

Effector
Globodera
Heterodera
Meloidogyne
Plant–nematode interaction
RNAi

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10.1007/978-1-62703-986-4_9

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abstract = "Secreted effectors represent the molecular interface between the nematode and its host plant. Studies that aimed at deciphering molecular plant–nematode interactions are hampered by technical hurdles that prevent the generation of transgenic nematodes. However, RNA interference (RNAi) has proven to be a valuable tool to specifically knock-down nematode effector genes, both ex planta and in planta. Plantmediated RNAi of nematode genes not only facilitates functional characterization of effectors but also lends itself to a novel control strategy against plant-parasitic nematodes. Here, we describe currently used methods to silence genes in plant-parasitic cyst and root-knot nematodes.",

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KW - Effector

KW - Globodera

KW - Heterodera

KW - Meloidogyne

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KW - RNAi

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Functional characterization of nematode effectors in plants

Abstract

Keywords

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