Functional analysis of pathogenicity proteins of the potato cyst nematode Globodera rostochiensis using RNAi

Q Chen, S Rehman, G Smant, JT Jones*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

114 Citations (Scopus)

Abstract

RNA interference (RNAi) has been used widely as a tool for examining gene function and a method that allows its use with plant-parasitic nematodes recently has been described. Here, we use a modified method to analyze the function of secreted beta-1,4, endoglucanases of the potato cyst nematode Globodera rostochiensis, the first in vivo functional analysis of a pathogenicity protein of a plant-parasitic nematode. Knockout of the beta-1,4, endoglucanases reduced the ability of the nematodes to invade roots. We also use RNAi to show that gr-ams-1, a secreted protein of the main sense organs (the amphids), is essential for host location.

Original languageEnglish
Pages (from-to)621-625
Number of pages5
JournalMolecular Plant-Microbe Interactions
Volume18
Issue number7
DOIs
Publication statusPublished - Jul 2005

Keywords

  • amphid-secreted protein
  • cellulase
  • gene silencing
  • invasion
  • DOUBLE-STRANDED-RNA
  • CAENORHABDITIS-ELEGANS
  • HETERODERA-GLYCINES
  • EXPRESSION
  • BETA-1,4-ENDOGLUCANASE
  • GENE
  • INTERFERENCE
  • PARASITISM
  • PALLIDA
  • AMPHIDS

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