Full-wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe, Kishan Dholakia, Peter R.T. Munro*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.
Original languageEnglish
Title of host publication2022 IEEE photonics conference (IPC)
Subtitle of host publicationproceedings
EditorsWeidong Zhou
Place of PublicationPiscataway, NJ
Number of pages2
ISBN (Electronic)9781665434874
ISBN (Print)9781665434881
Publication statusE-pub ahead of print - 14 Dec 2022

Publication series

NameIEEE photonics conference
ISSN (Print)2374-0140
ISSN (Electronic)2575-274X


  • Computational modeling
  • Microscopy
  • Scattering
  • Media
  • Optogenetics
  • Spatiotemporal phenomena
  • Photonics


Dive into the research topics of 'Full-wave modelling of two-photon microscopy with spatiotemporal focussing'. Together they form a unique fingerprint.

Cite this