Full-wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe; Kishan Dholakia; Peter R.T. Munro

doi:10.1109/ipc53466.2022.9975671

Full-wave modelling of two-photon microscopy with spatiotemporal focussing

Philip Wijesinghe, Kishan Dholakia, Peter R.T. Munro^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

Original language	English
Title of host publication	2022 IEEE photonics conference (IPC)
Subtitle of host publication	proceedings
Editors	Weidong Zhou
Place of Publication	Piscataway, NJ
Publisher	IEEE
Number of pages	2
ISBN (Electronic)	9781665434874
ISBN (Print)	9781665434881
DOIs	https://doi.org/10.1109/ipc53466.2022.9975671
Publication status	E-pub ahead of print - 14 Dec 2022

Publication series

Name	IEEE photonics conference
ISSN (Print)	2374-0140
ISSN (Electronic)	2575-274X

Keywords

Computational modeling
Microscopy
Scattering
Media
Optogenetics
Spatiotemporal phenomena
Photonics

Access to Document

10.1109/ipc53466.2022.9975671

IPC_spatiotemporal
Copyright © 2022 the Author(s). This work has been made available online in accordance with publisher policies or with permission. Permission for further reuse of this content should be sought from the publisher or the rights holder. This is the author created accepted manuscript following peer review and may differ slightly from the final published version. The final published version of this work is available at https://doi.org/10.1109/IPC53466.2022.9975671.
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Cite this

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title = "Full-wave modelling of two-photon microscopy with spatiotemporal focussing",

abstract = "Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.",

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author = "Philip Wijesinghe and Kishan Dholakia and Munro, {Peter R.T.}",

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language = "English",

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series = "IEEE photonics conference",

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AU - Wijesinghe, Philip

AU - Dholakia, Kishan

AU - Munro, Peter R.T.

PY - 2022/12/14

Y1 - 2022/12/14

N2 - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

AB - Spatiotemporal focussing has enabled widefield, axially confined multiphoton excitation. Its main advantage of an improved performance through scattering media, useful for deep imaging and optogenetics, however, has largely been evaluated empirically because of a lack of a suitable computational model. To overcome this we have developed a full-wave simulation describing two-photon microscopy image formation through scattering media with spatiotemporal focussing. This model provides full freedom to vary imaging parameters and observe quantities not generally accessible in experiment. We use this model to reveal new insights into the properties of spatiotemporal focussing in the presence of scattering media.

KW - Computational modeling

KW - Microscopy

KW - Scattering

KW - Media

KW - Optogenetics

KW - Spatiotemporal phenomena

KW - Photonics

UR - https://doi.org/10.1109/IPC53466.2022

U2 - 10.1109/ipc53466.2022.9975671

DO - 10.1109/ipc53466.2022.9975671

M3 - Conference contribution

SN - 9781665434881

T3 - IEEE photonics conference

BT - 2022 IEEE photonics conference (IPC)

A2 - Zhou, Weidong

PB - IEEE

CY - Piscataway, NJ

ER -

Full-wave modelling of two-photon microscopy with spatiotemporal focussing

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