Fluorometabolite biosynthesis: isotopically labelled glycerol incorporations into the antibiotic nucleocidin in Streptomyces calvus

Axel Bartholomé, Jeffrey E. Janso, Usa Reilly, David O'Hagan

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Deuterium and carbon-13 labelled glycerols have been fed to Streptomyces calvus fermentations and isotope incorporation into the fluorine containing antibiotic nucleocidin have been evaluated by 19F-NMR. A single deuterium atom was incorporated from [2H5]- and (R)-[2H2]- glycerol into C-5’ of the antibiotic, suggesting that an oxidation occurs at this carbon after ribose ring assembly from glycerol (pentose phosphate pathway), during nucleocidin biosynthesis.
Original languageEnglish
Pages (from-to)61-64
Number of pages4
JournalOrganic & Biomolecular Chemistry
Volume15
Issue number1
Early online date9 Nov 2016
DOIs
Publication statusPublished - 7 Jan 2017

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