FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases

Jinbo Zhu; Cally J.E. Haynes; Marion Kieffer; Jake L. Greenfield; Ryan D. Greenhalgh; Jonathan R. Nitschke; Ulrich F. Keyser

doi:10.1021/jacs.9b03566

Fe^II₄L₄ Tetrahedron Binds to Nonpaired DNA Bases

Jinbo Zhu, Cally J.E. Haynes, Marion Kieffer, Jake L. Greenfield, Ryan D. Greenhalgh, Jonathan R. Nitschke^*, Ulrich F. Keyser

^*Corresponding author for this work

School of Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A water-soluble self-assembled supramolecular Fe^II₄L₄ tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.

Original language	English
Pages (from-to)	11358-11362
Number of pages	5
Journal	Journal of the American Chemical Society
Volume	141
Issue number	29
DOIs	https://doi.org/10.1021/jacs.9b03566
Publication status	Published - 24 Jul 2019

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title = "FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases",

abstract = "A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.",

author = "Jinbo Zhu and Haynes, \{Cally J.E.\} and Marion Kieffer and Greenfield, \{Jake L.\} and Greenhalgh, \{Ryan D.\} and Nitschke, \{Jonathan R.\} and Keyser, \{Ulrich F.\}",

note = "Publisher Copyright: {\textcopyright} 2019 American Chemical Society.",

year = "2019",

month = jul,

day = "24",

doi = "10.1021/jacs.9b03566",

language = "English",

volume = "141",

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T1 - FeII4L4 Tetrahedron Binds to Nonpaired DNA Bases

AU - Zhu, Jinbo

AU - Haynes, Cally J.E.

AU - Kieffer, Marion

AU - Greenfield, Jake L.

AU - Greenhalgh, Ryan D.

AU - Nitschke, Jonathan R.

AU - Keyser, Ulrich F.

PY - 2019/7/24

Y1 - 2019/7/24

N2 - A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.

AB - A water-soluble self-assembled supramolecular FeII4L4 tetrahedron binds to single stranded DNA, mismatched DNA base pairs, and three-way DNA junctions. Binding of the coordination cage quenches fluorescent labels on the DNA strand, which provides an optical means to detect the interaction and allows the position of the binding site to be gauged with respect to the fluorescent label. Utilizing the quenching and binding properties of the coordination cage, we developed a simple and rapid detection method based on fluorescence quenching to detect unpaired bases in double-stranded DNA.

UR - https://www.scopus.com/pages/publications/85070485859

U2 - 10.1021/jacs.9b03566

DO - 10.1021/jacs.9b03566

M3 - Article

C2 - 31283214

AN - SCOPUS:85070485859

SN - 0002-7863

VL - 141

SP - 11358

EP - 11362

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 29

ER -

Fe^II₄L₄ Tetrahedron Binds to Nonpaired DNA Bases

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