Femtosecond optical transfection of cells: viability and efficiency

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Abstract

Photoporation is a rapidly expanding technique for the introduction of macromolecules into single cells. However, there remains no study into the true efficiency of this procedure. Here, we present a detailed analysis of transfection efficiency and cell viability for femtosecond optical transfection using a titanium sapphire laser at 800 nm. Photoporation of 4000 Chinese Hamster ovary cells was performed, representing the largest optical transfection study reported to date. We have investigated a range of laser fluences at the cell membrane and, at 1.2 mu J/cm(2), have found an average transfection efficiency of 50 +/- 10%. Contrary to recent literature, in which 100% efficiency is claimed, our measure of efficiency accounts for all irradiated cells, including those lost as a result of laser treatment, thereby providing a true biological measure of the technique. (c) 2006 Optical Society of America

Original languageEnglish
Pages (from-to)7125-7133
Number of pages9
JournalOptics Express
Volume14
Issue number16
DOIs
Publication statusPublished - 7 Aug 2006

Keywords

  • INFRARED LASER-PULSES
  • GENE DELIVERY
  • SINGLE CELLS
  • OPTOPORATION

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