Femtosecond cellular transfection using a nondiffracting light beam

X. Tsampoula, V. Garces-Chavez, M. Comrie, David James Stevenson, B. Agate, C. T. A. Brown, F. Gunn-Moore, K. Dholakia

Research output: Contribution to journalArticlepeer-review

143 Citations (Scopus)


The ability to permeate selectively the cell membrane and introduce therapeutic agents is a key goal in cell biology. Optical transfection is a powerful methodology but requires exact focusing due to the required two-photon power density. The authors use a Bessel beam that obviates the need to locate precisely the cell membrane, permitting two-photon excitation along a line leading to cell transfection. Assuming a minimum efficiency of 20%, the Bessel beam offers transfection at axial distances 20 times greater than that of its Gaussian equivalent. Furthermore, the authors demonstrate cell transfection beyond obstacles due to the self-healing nature of the Bessel beam. (C) 2007 American Institute of Physics.

Original languageEnglish
Article number053902
Number of pages3
JournalApplied Physics Letters
Issue number5
Publication statusPublished - 30 Jul 2007




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