Feeding increases MyoD and PCNA expression in myogenic progenitor cells of Notothenia coriiceps.

JC Brodeur, LS Peck, Ian Alistair Johnston

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34 Citations (Scopus)

Abstract

Oxygen consumption and ammonia excretion of the Antarctic fish Notothenia corikeps (18.4 cm L-F) increased respectively two and fourfold above fasting levels 24 h after feeding with a single meal of shrimps (5.5 to 7.5% of body mass), and remained elevated for 120 h. In fasted fish, c-met positive cells in the fast muscle represented 5.5% of the total number of myonuclei. The number of c-met positive cells staining for the proliferating cell nuclear antigen was increased by 60% both 24 and 96 h after the meal, while the number of cells expressing the myogenic transcription factor, MyoD, was increased by 20% after 24 h, and by 44% after 96 h. The total numbers of c-met positive cells and cells expressing myogenin were not significantly altered 96 h following feeding. The results are consistent with an activation of myogenic progenitor cells proliferation by feeding but suggest a relatively long cell cycle time. (C) 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1475-1485
Number of pages11
JournalJournal of Fish Biology
Volume60
DOIs
Publication statusPublished - Jun 2002

Keywords

  • myogenic precursor
  • satellite cell
  • SDA
  • MyoD
  • myogenin
  • Antarctic fish
  • MUSCLE SATELLITE CELLS
  • SKELETAL-MUSCLE
  • PROTEIN-SYNTHESIS
  • ANTARCTIC FISH
  • CHANNEL CATFISH
  • DYNAMIC ACTION
  • GROWTH
  • DEATH
  • TEMPERATURE
  • RICHARDSON

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