Failure to activate the IFN-beta promoter by a paramyxovirus lacking an interferon antagonist

M. J. Killip, D. F. Young, C. S. Ross, S. Chen, S. Goodbourn, R. E. Randall

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)

Abstract

It is generally thought that pathogen-associated molecular patterns (PAMPs) responsible for triggering interferon (IFN) induction are produced during virus replication and, to limit the activation of the IFN response by these PAMPs, viruses encode antagonists of IFN induction. Here we have studied the induction of IFN by parainfluenza virus type 5 (PIV5) at the single-cell level, using a cell line expressing GFP under the control of the IFN-beta promoter. We demonstrate that a recombinant PIV5 (termed PIV5-V Delta C) that lacks a functional V protein (the viral IFN antagonist) does not activate the IFN-beta promoter in the majority of infected cells. We conclude that viral PAMPs capable of activating the IFN induction cascade are not produced or exposed during the normal replication cycle of PIV5, and suggest instead that defective viruses are primarily responsible for inducing IFN during PIV5 infection in this system. (C) 2011 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalVirology
Volume415
Issue number1
Early online date22 Apr 2011
DOIs
Publication statusPublished - 20 Jun 2011

Keywords

  • Interferon
  • Interferon antagonist
  • Paramyxovirus
  • PIV5
  • Defective viruses
  • Vesicular stomatitis-virus
  • Cell antiviral responses
  • V-proteins
  • RIG-I
  • Regulatory factor-3
  • Induction
  • RNA
  • Simian-virus-5
  • Particles
  • Requires

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