Fading of auramine-stained mycobacterial smears and implications for external quality assurance

Jessica Minion, Shubhada Shenai, Viral Vadwai, Tejashree Tipnis, Christina Greenaway, Dick Menzies, Andrew Ramsay, Camilla Rodrigues, Madhukar Pai

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes in the proportion of positive slides in as little as 1 week.

Original languageEnglish
Pages (from-to)2024-6
Number of pages3
JournalJournal of Clinical Microbiology
Volume49
Issue number5
DOIs
Publication statusPublished - May 2011

Keywords

  • Bacteriological Techniques/methods
  • Benzophenoneidum/metabolism
  • Humans
  • Microscopy, Fluorescence/methods
  • Quality Assurance, Health Care
  • Staining and Labeling/methods

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