Extraction of nucleic acids from bone

Alun Hughes*, Tracy L. Stewart, Val Mann

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.

Original languageEnglish
Title of host publicationBone Research Protocols
EditorsMiep Helfrich, Stuart Ralston
Pages249-259
Number of pages11
DOIs
Publication statusPublished - 2012

Publication series

NameMethods in Molecular Biology
Volume816
ISSN (Print)1064-3745

Keywords

  • DNA extraction
  • DNA quantification
  • RNA extraction
  • RNA integrity
  • RNA quantification

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