Extraction of nucleic acids from bone

Alun Hughes; Tracy L. Stewart; Val Mann

doi:10.1007/978-1-61779-415-5_17

Extraction of nucleic acids from bone

Alun Hughes^*, Tracy L. Stewart, Val Mann

^*Corresponding author for this work

School of Medicine

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.

Original language	English
Title of host publication	Bone Research Protocols
Editors	Miep Helfrich, Stuart Ralston
Pages	249-259
Number of pages	11
DOIs	https://doi.org/10.1007/978-1-61779-415-5_17
Publication status	Published - 2012

Publication series

Name	Methods in Molecular Biology
Volume	816
ISSN (Print)	1064-3745

Keywords

DNA extraction
DNA quantification
RNA extraction
RNA integrity
RNA quantification

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10.1007/978-1-61779-415-5_17

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title = "Extraction of nucleic acids from bone",

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AU - Stewart, Tracy L.

AU - Mann, Val

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AB - Here, we present methods for extracting DNA and RNA from samples of whole bone tissue and culture bone cells and describe methods quantitative and qualitative measurement of the extracted nucleic acids. These protocols described provide high-quality nucleic acids suitable for downstream applications such as quantitative PCR and microarrays.

KW - DNA extraction

KW - DNA quantification

KW - RNA extraction

KW - RNA integrity

KW - RNA quantification

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U2 - 10.1007/978-1-61779-415-5_17

DO - 10.1007/978-1-61779-415-5_17

M3 - Chapter

C2 - 22130934

AN - SCOPUS:84555178774

SN - 9781617794148

T3 - Methods in Molecular Biology

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EP - 259

BT - Bone Research Protocols

A2 - Helfrich, Miep

A2 - Ralston, Stuart

ER -

Extraction of nucleic acids from bone

Abstract

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Keywords

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