Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..

J Seetharamappa; Muse Oke; Huanting Liu; Stephen McMahon; Kenneth Alan Johnson; Lester Carter; M Dorward; M Zawadzki; IM Overton; CA van Niekirk; S Graham; Catherine Helen Botting; Garry Lindsay Taylor; Malcolm Frederick White; GJ Barton; Peter John Coote; James Henderson Naismith

doi:10.1107/S1744309107019562

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..

J Seetharamappa, Muse Oke, Huanting Liu, Stephen McMahon, Kenneth Alan Johnson, Lester Carter, M Dorward, M Zawadzki, IM Overton, CA van Niekirk, S Graham, Catherine Helen Botting, Garry Lindsay Taylor, Malcolm Frederick White, GJ Barton, Peter John Coote, James Henderson Naismith

Research output: Contribution to journal › Article › peer-review

Abstract

Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 angstrom, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 angstrom resolution.

Original language	English
Pages (from-to)	452-456
Number of pages	5
Journal	Acta Crystallographica. Section F, Structural biology and crystallization communications
Volume	63
Issue number	5
DOIs	https://doi.org/10.1107/S1744309107019562
Publication status	Published - May 2007

Keywords

PROTEINS
MRSA

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10.1107/S1744309107019562

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BBSRC BBS/B/14426: SPORT
Naismith, J. (PI)
BBSRC
18/10/04 → 30/04/12
Project: Standard

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Seetharamappa, J., Oke, M., Liu, H., McMahon, S., Johnson, K. A., Carter, L., Dorward, M., Zawadzki, M., Overton, IM., van Niekirk, CA., Graham, S., Botting, C. H., Taylor, G. L., White, M. F., Barton, GJ., Coote, P. J., & Naismith, J. H. (2007). Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.. Acta Crystallographica. Section F, Structural biology and crystallization communications , 63(5), 452-456. https://doi.org/10.1107/S1744309107019562

Seetharamappa, J ; Oke, Muse ; Liu, Huanting et al. / Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.. In: Acta Crystallographica. Section F, Structural biology and crystallization communications . 2007 ; Vol. 63, No. 5. pp. 452-456.

@article{4c28ef4c103f4b3a87ceae11c2f84d8b,

title = "Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..",

abstract = "Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 angstrom, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 angstrom resolution.",

keywords = "PROTEINS, MRSA",

author = "J Seetharamappa and Muse Oke and Huanting Liu and Stephen McMahon and Johnson, {Kenneth Alan} and Lester Carter and M Dorward and M Zawadzki and IM Overton and {van Niekirk}, CA and S Graham and Botting, {Catherine Helen} and Taylor, {Garry Lindsay} and White, {Malcolm Frederick} and GJ Barton and Coote, {Peter John} and Naismith, {James Henderson}",

year = "2007",

month = may,

doi = "10.1107/S1744309107019562",

language = "English",

volume = "63",

pages = "452--456",

journal = "Acta Crystallographica. Section F, Structural biology and crystallization communications ",

issn = "1744-3091",

publisher = "Wiley-Blackwell",

number = "5",

}

Seetharamappa, J, Oke, M, Liu, H, McMahon, S, Johnson, KA, Carter, L, Dorward, M, Zawadzki, M, Overton, IM, van Niekirk, CA, Graham, S, Botting, CH, Taylor, GL , White, MF, Barton, GJ, Coote, PJ & Naismith, JH 2007, 'Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..', Acta Crystallographica. Section F, Structural biology and crystallization communications , vol. 63, no. 5, pp. 452-456. https://doi.org/10.1107/S1744309107019562

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.. / Seetharamappa, J; Oke, Muse; Liu, Huanting et al.
In: Acta Crystallographica. Section F, Structural biology and crystallization communications , Vol. 63, No. 5, 05.2007, p. 452-456.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..

AU - Seetharamappa, J

AU - Oke, Muse

AU - Liu, Huanting

AU - McMahon, Stephen

AU - Johnson, Kenneth Alan

AU - Carter, Lester

AU - Dorward, M

AU - Zawadzki, M

AU - Overton, IM

AU - van Niekirk, CA

AU - Graham, S

AU - Botting, Catherine Helen

AU - Taylor, Garry Lindsay

AU - White, Malcolm Frederick

AU - Barton, GJ

AU - Coote, Peter John

AU - Naismith, James Henderson

PY - 2007/5

Y1 - 2007/5

N2 - Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 angstrom, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 angstrom resolution.

AB - Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48 168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 angstrom, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 angstrom resolution.

KW - PROTEINS

KW - MRSA

UR - http://www.scopus.com/inward/record.url?scp=34248157100&partnerID=8YFLogxK

UR - http://ukpmc.ac.uk/abstract/MED/17565195

U2 - 10.1107/S1744309107019562

DO - 10.1107/S1744309107019562

M3 - Article

SN - 1744-3091

VL - 63

SP - 452

EP - 456

JO - Acta Crystallographica. Section F, Structural biology and crystallization communications

JF - Acta Crystallographica. Section F, Structural biology and crystallization communications

IS - 5

ER -

Seetharamappa J, Oke M, Liu H, McMahon S, Johnson KA, Carter L et al. Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.. Acta Crystallographica. Section F, Structural biology and crystallization communications . 2007 May;63(5):452-456. doi: 10.1107/S1744309107019562

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase..

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Keywords

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