Expression of Na+, K+-ATPase B subunit isoforms in the European Eel (Anguilla anguilla)

Gordon Cramb, Christopher Paul Cutler, IL Sanders

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Reverse transcriptase - polymerase chain reaction experiments utilising degenerate primers were used to demonstrate that the Na+,K+-ATPase beta 3 but not beta 2 isoform was present in brain tissue from the European eel (Anguilla anguilla). Two cDNA fragments were cloned and sequenced yielding 445 nucleotides of the Na+,K+-ATPase beta 3 isoform sequence. The putative amino acid sequence translated from this nucleotide sequence shared 58.0, 50.6 and 50.3% homology with previously published Na+,K+-ATPase "beta 3-like" isoform sequences from the clawed toad (Xenopus laevis), the chicken (Gallus domesticus) and the toad (Bufo marinus) respectively. The size of the Na+,K+-ATPase beta 3 mRNA transcript in eel tissues was demonstrated to be 2.4 Kb. Detectable levels of Na+,K+-ATPase beta 3 mRNA were only found in brain tissue. The tissue specific distribution of the beta 3 mRNA suggests that it plays little or no direct role in the process of osmoregulation in tissues such as the intestine or gill.

Original languageEnglish
Pages (from-to)371-376
Number of pages6
JournalFish Physiology and Biochemistry
Volume17
Publication statusPublished - Dec 1997

Keywords

  • Anguilla anguilla
  • beta 2 isoform
  • beta 3 isoform
  • brain
  • cDNA
  • eel
  • mRNA
  • Na+,K+-ATPase
  • nucleotide sequence
  • PCR
  • PRIMARY SEQUENCE
  • FUNCTIONAL EXPRESSION
  • TISSUE-SPECIFICITY
  • XENOPUS-LAEVIS
  • NA,K-ATPASE
  • TRANSPORT
  • SEAWATER
  • ATPASE
  • CELLS

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