Exploring the ultrashort pulse laser parameter space for membrane permeabilisation in mammalian cells.

Andrew Peter Rudhall, Maciej Antkowiak, Xanthi Tsampoula, Michael Mazilu, N Klaus Metzger, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
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Abstract

The use of ultrashort femtosecond pulsed lasers to effect membrane permeabilisation and initiate both optoinjection and transfection of cells has recently seen immense interest. We investigate femtosecond laser-induced membrane permeabilisation in mammalian cells as a function of pulse duration, pulse energy and number of pulses, by quantifying the efficiency of optoinjection for these parameters. Depending on pulse duration and pulse energy we identify two distinct membrane permeabilisation regimes. In the first regime a nonlinear dependence of order 3.4-9.6 is exhibited below a threshold peak power of at least 6 kW. Above this threshold peak power, the nonlinear dependence is saturated resulting in linear behaviour. This indicates that the membrane permeabilisation mechanism requires efficient multiphoton absorption to produce free electrons but once this process saturates, linear absorption dominates. Our experimental findings support a previously proposed theoretical model and provide a step towards the optimisation of laser-mediated gene delivery into mammalian cells.
Original languageEnglish
Pages (from-to)1-5
JournalNature Science Reports
Volume2
Issue number858
Early online date14 Nov 2012
DOIs
Publication statusPublished - 2012

Keywords

  • Ultrashort femtosecond pulsed lasers
  • Membrane permeabilisation
  • Mammalian cells

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