Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

Jeremy W. Dale*, Hasan Al-Ghusein, Salim Al-Hashmi, Philip Butcher, Anne L. Dickens, Francis Drobniewski, Ken J. Forbes, Stephen H. Gillespie, Dianie Lamprecht, Timothy D. McHugh, Richard Pitman, Nalin Rastogi, Andrew T. Smith, Christophe Sola, Hasan Yesilkaya

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)


Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

Original languageEnglish
Pages (from-to)2555-2562
Number of pages8
JournalJournal of Bacteriology
Issue number8
Publication statusPublished - 1 Apr 2003


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