Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

Jeremy W. Dale; Hasan Al-Ghusein; Salim Al-Hashmi; Philip Butcher; Anne L. Dickens; Francis Drobniewski; Ken J. Forbes; Stephen H. Gillespie; Dianie Lamprecht; Timothy D. McHugh; Richard Pitman; Nalin Rastogi; Andrew T. Smith; Christophe Sola; Hasan Yesilkaya

doi:10.1128/JB.185.8.2555-2562.2003

Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

Jeremy W. Dale^*, Hasan Al-Ghusein, Salim Al-Hashmi, Philip Butcher, Anne L. Dickens, Francis Drobniewski, Ken J. Forbes, Stephen H. Gillespie, Dianie Lamprecht, Timothy D. McHugh, Richard Pitman, Nalin Rastogi, Andrew T. Smith, Christophe Sola, Hasan Yesilkaya

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

Original language	English
Pages (from-to)	2555-2562
Number of pages	8
Journal	Journal of Bacteriology
Volume	185
Issue number	8
DOIs	https://doi.org/10.1128/JB.185.8.2555-2562.2003
Publication status	Published - 1 Apr 2003

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/JB.185.8.2555-2562.2003

Cite this

Dale, J. W., Al-Ghusein, H., Al-Hashmi, S., Butcher, P., Dickens, A. L., Drobniewski, F., Forbes, K. J., Gillespie, S. H., Lamprecht, D., McHugh, T. D., Pitman, R., Rastogi, N., Smith, A. T., Sola, C., & Yesilkaya, H. (2003). Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110. Journal of Bacteriology, 185(8), 2555-2562. https://doi.org/10.1128/JB.185.8.2555-2562.2003

@article{0b9c357c7bbe4e41ae03bd8bd724ddcc,

title = "Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110",

abstract = "Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.",

author = "Dale, {Jeremy W.} and Hasan Al-Ghusein and Salim Al-Hashmi and Philip Butcher and Dickens, {Anne L.} and Francis Drobniewski and Forbes, {Ken J.} and Gillespie, {Stephen H.} and Dianie Lamprecht and McHugh, {Timothy D.} and Richard Pitman and Nalin Rastogi and Smith, {Andrew T.} and Christophe Sola and Hasan Yesilkaya",

year = "2003",

month = apr,

day = "1",

doi = "10.1128/JB.185.8.2555-2562.2003",

language = "English",

volume = "185",

pages = "2555--2562",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "8",

}

Dale, JW, Al-Ghusein, H, Al-Hashmi, S, Butcher, P, Dickens, AL, Drobniewski, F, Forbes, KJ, Gillespie, SH, Lamprecht, D, McHugh, TD, Pitman, R, Rastogi, N, Smith, AT, Sola, C & Yesilkaya, H 2003, 'Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110', Journal of Bacteriology, vol. 185, no. 8, pp. 2555-2562. https://doi.org/10.1128/JB.185.8.2555-2562.2003

TY - JOUR

T1 - Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

AU - Dale, Jeremy W.

AU - Al-Ghusein, Hasan

AU - Al-Hashmi, Salim

AU - Butcher, Philip

AU - Dickens, Anne L.

AU - Drobniewski, Francis

AU - Forbes, Ken J.

AU - Gillespie, Stephen H.

AU - Lamprecht, Dianie

AU - McHugh, Timothy D.

AU - Pitman, Richard

AU - Rastogi, Nalin

AU - Smith, Andrew T.

AU - Sola, Christophe

AU - Yesilkaya, Hasan

PY - 2003/4/1

Y1 - 2003/4/1

N2 - Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

AB - Molecular typing of Mycobacterium tuberculosis by using IS6110 shows low discrimination when there are fewer than five copies of the insertion sequence. Using a collection of such isolates from a study of the epidemiology of tuberculosis in London, we have shown a substantial degree of congruence between IS6110 patterns and both spoligotype and PGRS type. This indicates that the IS6110 types mainly represent distinct families of strains rather than arising through the convergent insertion of IS6110 into favored positions. This is supported by identification of the genomic sites of the insertion of IS6110 in these strains. The combined data enable identification of the putative evolutionary relationships of these strains, comprising three lineages broadly associated with patients born in South Asia (India and Pakistan), Africa, and Europe, respectively. These lineages appear to be quite distinct from M. tuberculosis isolates with multiple copies of IS6110.

UR - http://www.scopus.com/inward/record.url?scp=0344838395&partnerID=8YFLogxK

U2 - 10.1128/JB.185.8.2555-2562.2003

DO - 10.1128/JB.185.8.2555-2562.2003

M3 - Article

C2 - 12670980

AN - SCOPUS:0344838395

SN - 0021-9193

VL - 185

SP - 2555

EP - 2562

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 8

ER -

Evolutionary relationships among strains of Mycobacterium tuberculosis with few copies of IS6110

Abstract

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this