Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models

D. M. O'Sullivan, C. Sander, R. J. Shorten, S. H. Gillespie, A. V. S. Hill, T. D. McHugh, H. McShane, E. Z. Tchilian*

*Corresponding author for this work

Research output: Contribution to journalLetterpeer-review

5 Citations (Scopus)

Abstract

Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. (C) 2007 Elsevier Ltd. All fights reserved.

Original languageEnglish
Pages (from-to)8203-8205
Number of pages3
JournalVaccine
Volume25
Issue number49
DOIs
Publication statusPublished - 28 Nov 2007

Keywords

  • Mycobacterium tuberculosis
  • mouse
  • CFU/ml
  • LOWENSTEIN-JENSEN MEDIUM
  • CLINICAL SPECIMENS
  • BACTEC MGIT-960
  • SYSTEM
  • TB
  • IDENTIFICATION
  • RECOVERY
  • COMPLEX
  • MEDIA

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