Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models

D. M. O'Sullivan; C. Sander; R. J. Shorten; S. H. Gillespie; A. V. S. Hill; T. D. McHugh; H. McShane; E. Z. Tchilian

doi:10.1016/j.vaccine.2007.09.065

Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models

D. M. O'Sullivan, C. Sander, R. J. Shorten, S. H. Gillespie, A. V. S. Hill, T. D. McHugh, H. McShane, E. Z. Tchilian^*

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

Abstract

Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. (C) 2007 Elsevier Ltd. All fights reserved.

Original language	English
Pages (from-to)	8203-8205
Number of pages	3
Journal	Vaccine
Volume	25
Issue number	49
DOIs	https://doi.org/10.1016/j.vaccine.2007.09.065
Publication status	Published - 28 Nov 2007

Keywords

Mycobacterium tuberculosis
mouse
CFU/ml
LOWENSTEIN-JENSEN MEDIUM
CLINICAL SPECIMENS
BACTEC MGIT-960
SYSTEM
TB
IDENTIFICATION
RECOVERY
COMPLEX
MEDIA

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.vaccine.2007.09.065

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@article{a73a57f597cd48bd8427b1bb39e9be2d,

title = "Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models",

abstract = "Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. (C) 2007 Elsevier Ltd. All fights reserved.",

keywords = "Mycobacterium tuberculosis, mouse, CFU/ml, LOWENSTEIN-JENSEN MEDIUM, CLINICAL SPECIMENS, BACTEC MGIT-960, SYSTEM, TB, IDENTIFICATION, RECOVERY, COMPLEX, MEDIA",

author = "O'Sullivan, {D. M.} and C. Sander and Shorten, {R. J.} and Gillespie, {S. H.} and Hill, {A. V. S.} and McHugh, {T. D.} and H. McShane and Tchilian, {E. Z.}",

year = "2007",

month = nov,

day = "28",

doi = "10.1016/j.vaccine.2007.09.065",

language = "English",

volume = "25",

pages = "8203--8205",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier",

number = "49",

}

TY - JOUR

T1 - Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models

AU - O'Sullivan, D. M.

AU - Sander, C.

AU - Shorten, R. J.

AU - Gillespie, S. H.

AU - Hill, A. V. S.

AU - McHugh, T. D.

AU - McShane, H.

AU - Tchilian, E. Z.

PY - 2007/11/28

Y1 - 2007/11/28

N2 - Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. (C) 2007 Elsevier Ltd. All fights reserved.

AB - Quantitation of bacterial load in tissues is essential for experimental investigation of Mycobacterium tuberculosis infection and immunity. We have used an automated liquid culture system to determine the number of colony forming units (CFU) in murine tissues and compared the results to those obtained by conventional plating on Middlebrook agar. There is an overall good correlation between results obtained by the two methods. Although less consistency and more contamination was observed in the automated liquid culture, the method is more sensitive, less labour intensive and allows the processing of large numbers of samples. (C) 2007 Elsevier Ltd. All fights reserved.

KW - Mycobacterium tuberculosis

KW - mouse

KW - CFU/ml

KW - LOWENSTEIN-JENSEN MEDIUM

KW - CLINICAL SPECIMENS

KW - BACTEC MGIT-960

KW - SYSTEM

KW - TB

KW - IDENTIFICATION

KW - RECOVERY

KW - COMPLEX

KW - MEDIA

U2 - 10.1016/j.vaccine.2007.09.065

DO - 10.1016/j.vaccine.2007.09.065

M3 - Letter

SN - 0264-410X

VL - 25

SP - 8203

EP - 8205

JO - Vaccine

JF - Vaccine

IS - 49

ER -

Evaluation of liquid culture for quantitation of Mycobacterium tuberculosis in murine models

Abstract

Keywords

UN SDGs

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