TY - JOUR
T1 - Evaluation of dual application of Photodynamic Therapy - PDT in Candida albicans
AU - Nunes, Iago P.F.
AU - Crugeira, Pedro J.L.
AU - Sampaio, Fernando J.P.
AU - de Oliveira, Susana C.P.S.
AU - Azevedo, Juliana M.
AU - Santos, Caio L.O.
AU - Soares, Luiz G.P.
AU - Samuel, Ifor D.W.
AU - Persheyev, Saydulla
AU - de Ameida, Paulo F.
AU - Pinheiro, Antônio L.B.
N1 - Funding: Grants from the Brazilian National Council for Scientific and Technological Development (CNPq) (No.304279/2018-8, No. 302753/2020-6 and Projeto PIBIT-UFBA n° 16476); Grants from the Bahia State Research Support Foundation) FAPESB (BOL0777/2016 and BOL0316/2018), and the British Council (SPA0-XGB-008).
PY - 2023/6/1
Y1 - 2023/6/1
N2 - This study aimed to evaluate, in vitro, the efficacy of photodynamic therapy - PDT using dimethyl methylene blue zinc chloride double salt (DMMB) and red LED light on planktonic cultures of Candida albicans. The tests were performed using the ATCC 90028 strain grown at 37°C for 24-h, according to a growth curve of C. albicans. The colonies were resuspended in sterile saline adjusted to a concentration of 2 × 108 cells / mL, with three experimental protocols being tested (Protocol 1, 2 and 3) with a fixed concentration of 750 ɳg/mL obtained through the IC50, and energy density 20 J/cm2. Protocol 1 was carried out using conventional PDT, Protocol 2 was applied double PDT in a single session, and Protocol 3 was applied double PDT in two sessions with a 24-h interval. The results showed logarithmic reductions of 3 (4.252575 ± 0.068526) and 4 logs (2.669533 ± 0.058592) of total fungal load in protocols 3 and 2 respectively in comparison to the Control (6.633547 ± 0.065384). Our results indicated that double application in a single session of PDT was the most effective approach for inhibiting the proliferation of Candida albicans (99.991 % inhibition).
AB - This study aimed to evaluate, in vitro, the efficacy of photodynamic therapy - PDT using dimethyl methylene blue zinc chloride double salt (DMMB) and red LED light on planktonic cultures of Candida albicans. The tests were performed using the ATCC 90028 strain grown at 37°C for 24-h, according to a growth curve of C. albicans. The colonies were resuspended in sterile saline adjusted to a concentration of 2 × 108 cells / mL, with three experimental protocols being tested (Protocol 1, 2 and 3) with a fixed concentration of 750 ɳg/mL obtained through the IC50, and energy density 20 J/cm2. Protocol 1 was carried out using conventional PDT, Protocol 2 was applied double PDT in a single session, and Protocol 3 was applied double PDT in two sessions with a 24-h interval. The results showed logarithmic reductions of 3 (4.252575 ± 0.068526) and 4 logs (2.669533 ± 0.058592) of total fungal load in protocols 3 and 2 respectively in comparison to the Control (6.633547 ± 0.065384). Our results indicated that double application in a single session of PDT was the most effective approach for inhibiting the proliferation of Candida albicans (99.991 % inhibition).
KW - C. albicans
KW - Taylor blue
KW - PDT
KW - Double application
KW - Photoinactivation
UR - https://www.scopus.com/pages/publications/85147888982
U2 - 10.1016/j.pdpdt.2023.103327
DO - 10.1016/j.pdpdt.2023.103327
M3 - Article
SN - 1572-1000
VL - 42
JO - Photodiagnosis and Photodynamic Therapy
JF - Photodiagnosis and Photodynamic Therapy
M1 - 103327
ER -