Enzyme Immobilisation using Siliceous Mesoporous Molecular Sieves

HHP Yiu, Paul Anthony Wright, Nigel Peter Botting

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199 Citations (Scopus)


The use of mesoporous molecular sieves in enzyme immobilisation has been studied. Three different types of mesoporous sieves (MCM-41, MCM-48 and SBA-15) were selected because of the differences in their pore dimensions and structures. Commercially available porous silica gel was chosen for comparison. The model enzyme chosen in this study was trypsin. The samples of immobilised trypsin were active For the hydrolysis of N-alpha -benzoyl-DL-arginine-4-nitro-anilide (BAPNA). The amount of enzyme adsorbed on the supports was found to be related to the pore size of the molecular sieves. Moreover. the pore size and the structure of the support also affected the activity of the supported enzymes. (C) 2001 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)763-768
Number of pages6
JournalMicroporous and Mesoporous Materials
Publication statusPublished - Jun 2001


  • enzyme immobilisation
  • trypsin
  • mesoporous molecular sieves
  • SBA-15
  • MCM-41


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