Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk; Kaley Allyn McCluskey; Sanya Aggarwal; Javier Tello; Kishan Dholakia

doi:10.1364/BOE.7.004021

Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk, Kaley Allyn McCluskey, Sanya Aggarwal, Javier Tello, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

Original language	English
Pages (from-to)	4021-4033
Journal	Biomedical Optics Express
Volume	7
Issue number	10
Early online date	14 Sept 2016
DOIs	https://doi.org/10.1364/BOE.7.004021
Publication status	Published - 1 Oct 2016

Access to Document

10.1364/BOE.7.004021Licence: CC BY

Nylk_2016_Enhacement_BOE_CC
Copyright 2016 the Author(s). Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License. Further distribution of this work must maintain attribution to the author(s) and the published article’s title, journal citation, and DOI.
Final published version, 4.81 MB

Leverhulme Trust Snr Research Fellowship: Accelerating progress for light sheet microscopy
Dholakia, K. (PI)
The Royal Society
1/09/15 → 31/08/16
Project: Fellowship
Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light
Dholakia, K. (PI), Krauss, T. F. (CoI) & Samuel, I. D. W. (CoI)
EPSRC
1/06/12 → 31/05/17
Project: Standard

Javier Ananda Tello
- jt65st-andrews.acuk
- School of Medicine - Lecturer in Pharmacology
- Centre for Biophotonics
- Biomedical Sciences Research Complex
- Institute of Behavioural and Neural Sciences
- Cellular Medicine Division
Person: Academic

Data underpinning: Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue
Nylk, J. (Creator), McCluskey, K. A. (Creator), Aggarwal, S. (Creator), Tello, J. (Creator) & Dholakia, K. (Creator), University of St Andrews, 20 Jun 2023
DOI: 10.17630/53f16b29-5d08-4aaa-ace3-0461b0dd0713
Dataset

Cite this

@article{089190f82f4546e58bf5fcee3382c908,

title = "Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue",

abstract = "We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.",

author = "Jonathan Nylk and McCluskey, {Kaley Allyn} and Sanya Aggarwal and Javier Tello and Kishan Dholakia",

note = "This work was supported by awards to KD from the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the {\textquoteright}BRAINS{\textquoteright} 600th anniversary appeal, and Dr. E. Killick. We would also like to thank The Northwood Trust and The RS Macdonald Charitable Trust for funding support (awarded to JAT). KD acknowledges support of a Royal Society Leverhulme Trust Senior Fellowship. ",

year = "2016",

month = oct,

day = "1",

doi = "10.1364/BOE.7.004021",

language = "English",

volume = "7",

pages = "4021--4033",

journal = "Biomedical Optics Express",

issn = "2156-7085",

publisher = "OPTICAL SOC AMER",

number = "10",

}

TY - JOUR

T1 - Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

AU - Nylk, Jonathan

AU - McCluskey, Kaley Allyn

AU - Aggarwal, Sanya

AU - Tello, Javier

AU - Dholakia, Kishan

N1 - This work was supported by awards to KD from the UK Engineering and Physical Sciences Research Council under grant EP/J01771X/1, the ’BRAINS’ 600th anniversary appeal, and Dr. E. Killick. We would also like to thank The Northwood Trust and The RS Macdonald Charitable Trust for funding support (awarded to JAT). KD acknowledges support of a Royal Society Leverhulme Trust Senior Fellowship.

PY - 2016/10/1

Y1 - 2016/10/1

N2 - We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

AB - We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

U2 - 10.1364/BOE.7.004021

DO - 10.1364/BOE.7.004021

M3 - Article

SN - 2156-7085

VL - 7

SP - 4021

EP - 4033

JO - Biomedical Optics Express

JF - Biomedical Optics Express

IS - 10

ER -

Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

Abstract

Access to Document

Fingerprint

Projects

Leverhulme Trust Snr Research Fellowship: Accelerating progress for light sheet microscopy

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

Profiles

Javier Ananda Tello

Datasets

Data underpinning: Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

Cite this