Enhanced optical manipulation of cells using anti-reflection coated microparticles

Derek Craig; Alison McDonald; Michael Mazilu; Helen Anne Rendall; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1021/acsphotonics.5b00178

Enhanced optical manipulation of cells using anti-reflection coated microparticles

Derek Craig, Alison McDonald, Michael Mazilu, Helen Anne Rendall, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

We demonstrate the use of anti-reflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate both CHO-K1 and HL60 cell lines with AR coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50um/s were recorded for only 33mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI) which verified no disruption to the cell cycle in the presence of AR microparticles.

Original language	English
Pages (from-to)	1403-1409
Journal	ACS Photonics
Volume	2
Issue number	10
Early online date	11 Sept 2015
DOIs	https://doi.org/10.1021/acsphotonics.5b00178
Publication status	Published - 2015

Keywords

Optical trapping
Dielectric tagging
Anti-reflection
Microparticles
Biophotonics
Cell viability

Access to Document

10.1021/acsphotonics.5b00178

Mazilu_2015_ACSP_Enhanced_AM
Copyright © 2015 American Chemical Society. This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Photonics, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://dx.doi.org/10.1021/acsphotonics.5b00178
Accepted author manuscript, 807 KB

Shaped light at the interface: Shaped Light at the Interface
Dholakia, K. (PI), Brown, C. T. A. (CoI), Di Falco, A. (CoI) & Mazilu, M. (CoI)
EPSRC
1/09/14 → 31/08/19
Project: Standard
Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light
Dholakia, K. (PI), Krauss, T. F. (CoI) & Samuel, I. D. W. (CoI)
EPSRC
1/06/12 → 31/05/17
Project: Standard

EigenOptics v1.00
Mazilu, M. (Creator), University of St Andrews, 2015
DOI: 10.17630/F039C29B-12FE-4B4B-9B0B-907C72634AD8
Dataset

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Data Underpinning - Enhanced optical manipulation of cells using anti-reflection coated microparticles
Craig, D. (Creator), McDonald, A. (Creator), Mazilu, M. (Creator), Rendall, H. A. (Creator), Gunn-Moore, F. J. (Creator) & Dholakia, K. (Creator), University of St Andrews, 2015
DOI: 10.17630/6B2CC9F9-90F2-4B83-9990-35CFF3FF31E5
Dataset

File

Cite this

@article{1082ffcf9bd9480db4d2c5fa665c5442,

title = "Enhanced optical manipulation of cells using anti-reflection coated microparticles",

abstract = "We demonstrate the use of anti-reflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate both CHO-K1 and HL60 cell lines with AR coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50um/s were recorded for only 33mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI) which verified no disruption to the cell cycle in the presence of AR microparticles. ",

keywords = "Optical trapping, Dielectric tagging, Anti-reflection, Microparticles, Biophotonics, Cell viability",

author = "Derek Craig and Alison McDonald and Michael Mazilu and Rendall, {Helen Anne} and Gunn-Moore, {Frank J} and Kishan Dholakia",

note = "The authors thank the UK Engineering and Physical Sciences Research Council under grants EP/J01771X/1 and EP/M000869/1, the University of St Andrews, the BRAINS 600th anniversary appeal and Dr. Killick for funding.",

year = "2015",

doi = "10.1021/acsphotonics.5b00178",

language = "English",

volume = "2",

pages = "1403--1409",

journal = "ACS Photonics",

publisher = "American Chemical Society (ACS)",

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T1 - Enhanced optical manipulation of cells using anti-reflection coated microparticles

AU - Craig, Derek

AU - McDonald, Alison

AU - Mazilu, Michael

AU - Rendall, Helen Anne

AU - Gunn-Moore, Frank J

AU - Dholakia, Kishan

N1 - The authors thank the UK Engineering and Physical Sciences Research Council under grants EP/J01771X/1 and EP/M000869/1, the University of St Andrews, the BRAINS 600th anniversary appeal and Dr. Killick for funding.

PY - 2015

Y1 - 2015

N2 - We demonstrate the use of anti-reflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate both CHO-K1 and HL60 cell lines with AR coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50um/s were recorded for only 33mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI) which verified no disruption to the cell cycle in the presence of AR microparticles.

AB - We demonstrate the use of anti-reflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate both CHO-K1 and HL60 cell lines with AR coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50um/s were recorded for only 33mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI) which verified no disruption to the cell cycle in the presence of AR microparticles.

KW - Optical trapping

KW - Dielectric tagging

KW - Anti-reflection

KW - Microparticles

KW - Biophotonics

KW - Cell viability

U2 - 10.1021/acsphotonics.5b00178

DO - 10.1021/acsphotonics.5b00178

M3 - Article

VL - 2

SP - 1403

EP - 1409

JO - ACS Photonics

JF - ACS Photonics

IS - 10

ER -

Enhanced optical manipulation of cells using anti-reflection coated microparticles

Abstract

Keywords

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Projects

Shaped light at the interface: Shaped Light at the Interface

Challening the Limits of Photonics: stru: Challenging the Limits of Photonics: Structured Light

Datasets

EigenOptics v1.00

Data Underpinning - Enhanced optical manipulation of cells using anti-reflection coated microparticles

Cite this