Enhanced Cell Transfection using sub-wavelength focused optical eigenmode beams

Xanthi Tsampoula; Michael Mazilu; Tom Vettenburg; Frank J Gunn-Moore; Kishan Dholakia

doi:10.1364/PRJ.1.000042

Enhanced Cell Transfection using sub-wavelength focused optical eigenmode beams

Xanthi Tsampoula, Michael Mazilu, Tom Vettenburg, Frank J Gunn-Moore, Kishan Dholakia

Research output: Contribution to journal › Article › peer-review

Abstract

We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection. © 2013 Chinese Laser Press

Original language	English
Pages (from-to)	42-46
Number of pages	5
Journal	Photonics Research
Volume	1
Issue number	1
DOIs	https://doi.org/10.1364/PRJ.1.000042
Publication status	Published - 11 Jun 2013

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10.1364/PRJ.1.000042

Making Light deliver:Translation of: Making light deliver translation of methods of photoportions
Dholakia, K. (PI)
EPSRC
1/10/10 → 30/09/14
Project: Standard

Cite this

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title = "Enhanced Cell Transfection using sub-wavelength focused optical eigenmode beams",

abstract = "We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection. {\textcopyright} 2013 Chinese Laser Press",

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AU - Dholakia, Kishan

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AB - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection. © 2013 Chinese Laser Press

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DO - 10.1364/PRJ.1.000042

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Enhanced Cell Transfection using sub-wavelength focused optical eigenmode beams

Abstract

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Making Light deliver:Translation of: Making light deliver translation of methods of photoportions

Cite this