Abstract
To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis.
Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression.
Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment.
The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.
| Original language | English |
|---|---|
| Pages (from-to) | 1199-1202 |
| Number of pages | 4 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 62 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2008 |
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
Keywords
- PSEUDOMONAS-AERUGINOSA
- PULMONARY TUBERCULOSIS
- STAPHYLOCOCCUS-AUREUS
- GENE-EXPRESSION
- SOS RESPONSE
- RESISTANCE
- FLUOROQUINOLONES
- 4-QUINOLONES
- INDUCTION
- SURVIVAL
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