Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin

D. M. O'Sullivan, J. Hinds, P. D. Butcher, Stephen Henry Gillespie, T. D. McHugh

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)

Abstract

To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis.

Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression.

Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment.

The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.

Original languageEnglish
Pages (from-to)1199-1202
Number of pages4
JournalJournal of Antimicrobial Chemotherapy
Volume62
Issue number6
DOIs
Publication statusPublished - Dec 2008

Keywords

  • PSEUDOMONAS-AERUGINOSA
  • PULMONARY TUBERCULOSIS
  • STAPHYLOCOCCUS-AUREUS
  • GENE-EXPRESSION
  • SOS RESPONSE
  • RESISTANCE
  • FLUOROQUINOLONES
  • 4-QUINOLONES
  • INDUCTION
  • SURVIVAL

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