Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin

D. M. O'Sullivan; J. Hinds; P. D. Butcher; Stephen Henry Gillespie; T. D. McHugh

doi:10.1093/jac/dkn387

Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin

D. M. O'Sullivan, J. Hinds, P. D. Butcher, Stephen Henry Gillespie, T. D. McHugh

Research output: Contribution to journal › Article › peer-review

Abstract

To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis.

Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression.

Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment.

The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.

Original language	English
Pages (from-to)	1199-1202
Number of pages	4
Journal	Journal of Antimicrobial Chemotherapy
Volume	62
Issue number	6
DOIs	https://doi.org/10.1093/jac/dkn387
Publication status	Published - Dec 2008

Keywords

PSEUDOMONAS-AERUGINOSA
PULMONARY TUBERCULOSIS
STAPHYLOCOCCUS-AUREUS
GENE-EXPRESSION
SOS RESPONSE
RESISTANCE
FLUOROQUINOLONES
4-QUINOLONES
INDUCTION
SURVIVAL

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1093/jac/dkn387

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title = "Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin",

abstract = "To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis. Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression. Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment. The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.",

keywords = "PSEUDOMONAS-AERUGINOSA, PULMONARY TUBERCULOSIS, STAPHYLOCOCCUS-AUREUS, GENE-EXPRESSION, SOS RESPONSE, RESISTANCE, FLUOROQUINOLONES, 4-QUINOLONES, INDUCTION, SURVIVAL",

author = "O'Sullivan, {D. M.} and J. Hinds and Butcher, {P. D.} and Gillespie, {Stephen Henry} and McHugh, {T. D.}",

year = "2008",

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doi = "10.1093/jac/dkn387",

language = "English",

volume = "62",

pages = "1199--1202",

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publisher = "Oxford University Press",

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TY - JOUR

T1 - Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin

AU - O'Sullivan, D. M.

AU - Hinds, J.

AU - Butcher, P. D.

AU - Gillespie, Stephen Henry

AU - McHugh, T. D.

PY - 2008/12

Y1 - 2008/12

N2 - To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis. Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression. Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment. The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.

AB - To investigate how the SOS response, an error-prone DNA repair pathway, is expressed following subinhibitory quinolone treatment of Mycobacterium tuberculosis. Genome-wide expression profiling followed by quantitative RT (qRT)-PCR was used to study the effect of ciprofloxacin on M. tuberculosis gene expression. Microarray analysis showed that 16/110 genes involved in DNA protection, repair and recombination were up-regulated. There appeared to be a lack of downstream genes involved in the SOS response. qRT-PCR detected an induction of lexA and recA after 4 h and of dnaE2 after 24 h of subinhibitory treatment. The pattern of gene expression observed following subinhibitory quinolone treatment differed from that induced after other DNA-damaging agents (e.g. mitomycin C). The expression of the DnaE2 polymerase response was significantly delayed following subinhibitory quinolone exposure.

KW - PSEUDOMONAS-AERUGINOSA

KW - PULMONARY TUBERCULOSIS

KW - STAPHYLOCOCCUS-AUREUS

KW - GENE-EXPRESSION

KW - SOS RESPONSE

KW - RESISTANCE

KW - FLUOROQUINOLONES

KW - 4-QUINOLONES

KW - INDUCTION

KW - SURVIVAL

U2 - 10.1093/jac/dkn387

DO - 10.1093/jac/dkn387

M3 - Article

SN - 0305-7453

VL - 62

SP - 1199

EP - 1202

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 6

ER -

Mycobacterium tuberculosis DNA repair in response to subinhibitory concentrations of ciprofloxacin

Abstract

Keywords

UN SDGs

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