BIGYIN,an orthologue of human and yeast FIS1 genes functions in the control of mitochondrial size and number in Arabidopsis thaliana--

I Scott, Alyson Kim Tobin, David Logan

Research output: Contribution to journalArticlepeer-review

69 Citations (Scopus)

Abstract

Reverse-genetics was used to evaluate the role of an Arabidopsis homologue of the human and yeast FIS1 genes, which are both involved in mitochondrial fission. Two independent T-DNA insertion mutants of gene At3g57090 were identified and genetically transformed to express mitochondria-targeted GFP to enable visualization of mitochondria in vivo. Plants homozygous for either of the recessive T-DNA mutant alleles, termed bigyin1-1 (bgy1-1) and bigyin1-2 (bgy1-2), displayed an abnormal mitochondrial morphology. Disruption of BIGYIN leads to a reduced number of mitochondria per cell, coupled to a large increase in the size of individual mitochondria, relative to wild-type. It is concluded that BIGYIN is an Arabidopsis FIS orthologue and is part of the Arabidopsis mitochondrial division apparatus.

Original languageEnglish
Pages (from-to)1275-1280
Number of pages6
JournalJournal of Experimental Botany
Volume57
Issue number6
DOIs
Publication statusPublished - Mar 2006

Keywords

  • dynamin
  • FIS1
  • mitochondria
  • mitochondrial division
  • morphology
  • organelle fission
  • DYNAMIN-RELATED GTPASE
  • PROTEIN HFIS1
  • DIVISION
  • FISSION
  • DNM1P
  • MORPHOLOGY
  • CELLS
  • ADL2B
  • APOPTOSIS
  • COMPONENT

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