Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

Frøydis Sved Skottvoll; Frederik André Hansen; Sean Harrison; Ida Sneis Boger; Ago Mrsa; Magnus Saed Restan; Matthias Stein; Elsa Lundanes; Stig Pedersen-Bjergaard; Aleksandra Aizenshtadt; Stefan Krauss; Gareth Sullivan; Inger Lise Bogen; Steven Ray Wilson

doi:10.1021/acs.analchem.0c05082

Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

Frøydis Sved Skottvoll, Frederik André Hansen, Sean Harrison, Ida Sneis Boger, Ago Mrsa, Magnus Saed Restan, Matthias Stein, Elsa Lundanes, Stig Pedersen-Bjergaard, Aleksandra Aizenshtadt, Stefan Krauss, Gareth Sullivan, Inger Lise Bogen, Steven Ray Wilson^*

^*Corresponding author for this work

School of Medicine

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.

Original language	English
Pages (from-to)	3576-3585
Number of pages	10
Journal	Analytical Chemistry
Volume	93
Issue number	7
DOIs	https://doi.org/10.1021/acs.analchem.0c05082
Publication status	Published - 23 Feb 2021

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Skottvoll, F. S., Hansen, F. A., Harrison, S., Boger, I. S., Mrsa, A., Restan, M. S., Stein, M., Lundanes, E., Pedersen-Bjergaard, S., Aizenshtadt, A., Krauss, S., Sullivan, G., Bogen, I. L., & Wilson, S. R. (2021). Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies. Analytical Chemistry, 93(7), 3576-3585. https://doi.org/10.1021/acs.analchem.0c05082

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title = "Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies",

abstract = "Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.",

author = "Skottvoll, {Fr{\o}ydis Sved} and Hansen, {Frederik Andr{\'e}} and Sean Harrison and Boger, {Ida Sneis} and Ago Mrsa and Restan, {Magnus Saed} and Matthias Stein and Elsa Lundanes and Stig Pedersen-Bjergaard and Aleksandra Aizenshtadt and Stefan Krauss and Gareth Sullivan and Bogen, {Inger Lise} and Wilson, {Steven Ray}",

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Skottvoll, FS, Hansen, FA, Harrison, S, Boger, IS, Mrsa, A, Restan, MS, Stein, M, Lundanes, E, Pedersen-Bjergaard, S, Aizenshtadt, A, Krauss, S, Sullivan, G, Bogen, IL & Wilson, SR 2021, 'Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies', Analytical Chemistry, vol. 93, no. 7, pp. 3576-3585. https://doi.org/10.1021/acs.analchem.0c05082

TY - JOUR

T1 - Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

AU - Skottvoll, Frøydis Sved

AU - Hansen, Frederik André

AU - Harrison, Sean

AU - Boger, Ida Sneis

AU - Mrsa, Ago

AU - Restan, Magnus Saed

AU - Stein, Matthias

AU - Lundanes, Elsa

AU - Pedersen-Bjergaard, Stig

AU - Aizenshtadt, Aleksandra

AU - Krauss, Stefan

AU - Sullivan, Gareth

AU - Bogen, Inger Lise

AU - Wilson, Steven Ray

PY - 2021/2/23

Y1 - 2021/2/23

N2 - Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.

AB - Liver organoids are emerging tools for precision drug development and toxicity screening. We demonstrate that electromembrane extraction (EME) based on electrophoresis across an oil membrane is suited for segregating selected organoid-derived drug metabolites prior to mass spectrometry (MS)-based measurements. EME allowed drugs and drug metabolites to be separated from cell medium components (albumin, etc.) that could interfere with subsequent measurements. Multiwell EME (parallel-EME) holding 100 μL solutions allowed for simple and repeatable monitoring of heroin phase I metabolism kinetics. Organoid parallel-EME extracts were compatible with ultrahigh-performance liquid chromatography (UHPLC) used to separate the analytes prior to detection. Taken together, liver organoids are well-matched with EME followed by MS-based measurements.

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SP - 3576

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JO - Analytical Chemistry

JF - Analytical Chemistry

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ER -

Electromembrane Extraction and Mass Spectrometry for Liver Organoid Drug Metabolism Studies

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