TY - JOUR
T1 - Electrically tunable lens speeds up 3D orbital tracking
AU - Annibale, Paolo
AU - Dvornikov, Alexander
AU - Gratton, Enrico
N1 - Publisher Copyright:
� 2015 Optical Society of America.
PY - 2015/5/21
Y1 - 2015/5/21
N2 - 3D orbital particle tracking is a versatile and effective microscopy technique that allows following fast moving fluorescent objects within living cells and reconstructing complex 3D shapes using laser scanning microscopes. We demonstrated notable improvements in the range, speed and accuracy of 3D orbital particle tracking by replacing commonly used piezoelectric stages with Electrically Tunable Lens (ETL) that eliminates mechanical movement of objective lenses. This allowed tracking and reconstructing shape of structures extending 500 microns in the axial direction. Using the ETL, we tracked at high speed fluorescently labeled genomic loci within the nucleus of living cells with unprecedented temporal resolution of 8ms using a 1.42NA oil-immersion objective. The presented technology is cost effective and allows easy upgrade of scanning microscopes for fast 3D orbital tracking.
AB - 3D orbital particle tracking is a versatile and effective microscopy technique that allows following fast moving fluorescent objects within living cells and reconstructing complex 3D shapes using laser scanning microscopes. We demonstrated notable improvements in the range, speed and accuracy of 3D orbital particle tracking by replacing commonly used piezoelectric stages with Electrically Tunable Lens (ETL) that eliminates mechanical movement of objective lenses. This allowed tracking and reconstructing shape of structures extending 500 microns in the axial direction. Using the ETL, we tracked at high speed fluorescently labeled genomic loci within the nucleus of living cells with unprecedented temporal resolution of 8ms using a 1.42NA oil-immersion objective. The presented technology is cost effective and allows easy upgrade of scanning microscopes for fast 3D orbital tracking.
UR - http://www.scopus.com/inward/record.url?scp=84945927846&partnerID=8YFLogxK
U2 - 10.1364/BOE.6.002181
DO - 10.1364/BOE.6.002181
M3 - Article
AN - SCOPUS:84945927846
SN - 2156-7085
VL - 6
SP - 2181
EP - 2190
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 6
ER -