Efficient RFLP-based protocol for routine authentication of Drosophila

Melika Ghasemi Shiran; Nick P. Bailey; Lauren McCann; Natalia Rivera-Rincón; Emma Saurette; Laurie S. Stevison

doi:10.17912/MICROPUB.BIOLOGY.001949

Efficient RFLP-based protocol for routine authentication of Drosophila

Melika Ghasemi Shiran, Nick P. Bailey, Lauren McCann, Natalia Rivera-Rincón, Emma Saurette, Laurie S. Stevison^*

^*Corresponding author for this work

School of Biology

Research output: Contribution to journal › Article › peer-review

Abstract

Authentication of strains is important for preventing genetic contamination before any experiment, which can compromise reproducibility and lead to misleading results. Here, we developed an approach that combines computational single nucleotide polymorphism (SNP) identification with molecular validation using restriction fragment length polymorphisms (RFLPs). This workflow enables rapid and precise confirmation of strains in an inexpensive, reproducible, and easily adaptable way for long-term stock maintenance across laboratories. We apply this protocol to Drosophila melanogaster from the Drosophila Genome Resource Panel (DGRP), which are commonly used in fruit fly research, providing a reliable context for ensuring the integrity of Drosophila genetic resources.

Original language	English
Article number	001949
Pages (from-to)	1-5
Number of pages	5
Journal	microPublication Biology
Volume	2026
DOIs	https://doi.org/10.17912/MICROPUB.BIOLOGY.001949
Publication status	Published - 21 Jan 2026

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Shiran-et-al-2026-Efficient-RFLP-based-protocol-Micropubbiology-001949-CCBY
Copyright: © 2026 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Cite this

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title = "Efficient RFLP-based protocol for routine authentication of Drosophila",

abstract = "Authentication of strains is important for preventing genetic contamination before any experiment, which can compromise reproducibility and lead to misleading results. Here, we developed an approach that combines computational single nucleotide polymorphism (SNP) identification with molecular validation using restriction fragment length polymorphisms (RFLPs). This workflow enables rapid and precise confirmation of strains in an inexpensive, reproducible, and easily adaptable way for long-term stock maintenance across laboratories. We apply this protocol to Drosophila melanogaster from the Drosophila Genome Resource Panel (DGRP), which are commonly used in fruit fly research, providing a reliable context for ensuring the integrity of Drosophila genetic resources.",

author = "Shiran, \{Melika Ghasemi\} and Bailey, \{Nick P.\} and Lauren McCann and Natalia Rivera-Rinc{\'o}n and Emma Saurette and Stevison, \{Laurie S.\}",

note = "Funding: Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM147501. Supported by National Institute of General Medical Sciences (United States) R35GM147501 to LS Stevison.",

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doi = "10.17912/MICROPUB.BIOLOGY.001949",

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AU - McCann, Lauren

AU - Rivera-Rincón, Natalia

AU - Saurette, Emma

AU - Stevison, Laurie S.

N1 - Funding: Research reported in this publication was supported by the National Institute of General Medical Sciences of the National Institutes of Health under Award Number R35GM147501. Supported by National Institute of General Medical Sciences (United States) R35GM147501 to LS Stevison.

PY - 2026/1/21

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Efficient RFLP-based protocol for routine authentication of Drosophila

Abstract

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