Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses

A Kohl, A Bridgen, E Dunn, J N Barr, Richard Michael Elliott

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The genome of Bunyamwera virus (BUN) consists of three segments of single-stranded RNA of negative polarity. The smallest segment, S, encodes the N protein and a nonstructural protein called NSs. We recently described a mutant virus (BUNdelNSs) that does not express NSs but overexpresses N and grows to lower titres than wild-type (wt) BUN. Here we report a BUNdelNSs variant that expresses lower levels of N protein and grows to higher titres. Sequencing of the 3' and 5' termini of the BUNdelNSs S RNA segment and analysis using a minireplicon system show that the N overexpressing phenotype results from a single nucleotide substitution at position 16 in the 3' terminus. This mutation could also be detected in wtBUN populations, and was isolated by plaquing a 'wt' variant carrying the mutation. This variant was found to express increased N and NSs levels, and grew to lower titres than wtBUN.

Original languageEnglish
Pages (from-to)789-793
Number of pages5
JournalJournal of General Virology
Volume84
Issue number4
DOIs
Publication statusPublished - Apr 2003

Keywords

  • NONSTRUCTURAL PROTEIN NSS
  • RNA
  • BUNYAVIRUS
  • EXPRESSION
  • RESCUE
  • SYSTEM

Fingerprint

Dive into the research topics of 'Effects of a point mutation in the 3' end of the S genome segment of naturally occurring and engineered Bunyamwera viruses'. Together they form a unique fingerprint.

Cite this