Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

Myat Htut Nyunt, Myat Phone Kyaw, Kyaw Zin Thant, Thinzer Shein, Soe Soe Han, Ni Ni Zaw, Jin-Hee Han, Seong-Kyun Lee, Fauzi Muh, Jung-Yeon Kim, Shin-Hyeong Cho, Sang-Eun Lee, Eun-Jeong Yang, Chulhun L. Chang, Eun-Taek Han*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalKorean Journal of Parasitology
Volume54
Issue number3
DOIs
Publication statusPublished - 30 Jun 2016

Keywords

  • Plasmodium falciparum
  • Plasmodium vivax
  • Malaria
  • Pooling strategy
  • Low-transmission setting
  • Nested PCR

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