Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

Myat Htut Nyunt; Myat Phone Kyaw; Kyaw Zin Thant; Thinzer Shein; Soe Soe Han; Ni Ni Zaw; Jin-Hee Han; Seong-Kyun Lee; Fauzi Muh; Jung-Yeon Kim; Shin-Hyeong Cho; Sang-Eun Lee; Eun-Jeong Yang; Chulhun L. Chang; Eun-Taek Han

doi:10.3347/kjp.2016.54.3.253

Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

Myat Htut Nyunt, Myat Phone Kyaw, Kyaw Zin Thant, Thinzer Shein, Soe Soe Han, Ni Ni Zaw, Jin-Hee Han, Seong-Kyun Lee, Fauzi Muh, Jung-Yeon Kim, Shin-Hyeong Cho, Sang-Eun Lee, Eun-Jeong Yang, Chulhun L. Chang, Eun-Taek Han^*

^*Corresponding author for this work

School of Medicine

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Abstract

In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.

Original language	English
Pages (from-to)	253-259
Number of pages	7
Journal	Korean Journal of Parasitology
Volume	54
Issue number	3
DOIs	https://doi.org/10.3347/kjp.2016.54.3.253
Publication status	Published - 30 Jun 2016

Keywords

Plasmodium falciparum
Plasmodium vivax
Malaria
Pooling strategy
Low-transmission setting
Nested PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3347/kjp.2016.54.3.253Licence: CC BY-NC

Hynut-2016-Effective-high-KJP-54-3-253-CC
© 2016, Korean Society for Parasitology and Tropical Medicine. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 939 KBLicence: CC BY-NC

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Nyunt, M. H., Kyaw, M. P., Thant, K. Z., Shein, T., Han, S. S., Zaw, N. N., Han, J.-H., Lee, S.-K., Muh, F., Kim, J.-Y., Cho, S.-H., Lee, S.-E., Yang, E.-J., Chang, C. L., & Han, E.-T. (2016). Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings. Korean Journal of Parasitology, 54(3), 253-259. https://doi.org/10.3347/kjp.2016.54.3.253

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abstract = "In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.",

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author = "Nyunt, {Myat Htut} and Kyaw, {Myat Phone} and Thant, {Kyaw Zin} and Thinzer Shein and Han, {Soe Soe} and Zaw, {Ni Ni} and Jin-Hee Han and Seong-Kyun Lee and Fauzi Muh and Jung-Yeon Kim and Shin-Hyeong Cho and Sang-Eun Lee and Eun-Jeong Yang and Chang, {Chulhun L.} and Eun-Taek Han",

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language = "English",

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Nyunt, MH, Kyaw, MP, Thant, KZ, Shein, T, Han, SS, Zaw, NN, Han, J-H, Lee, S-K, Muh, F, Kim, J-Y, Cho, S-H, Lee, S-E, Yang, E-J, Chang, CL & Han, E-T 2016, 'Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings', Korean Journal of Parasitology, vol. 54, no. 3, pp. 253-259. https://doi.org/10.3347/kjp.2016.54.3.253

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T1 - Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

AU - Nyunt, Myat Htut

AU - Kyaw, Myat Phone

AU - Thant, Kyaw Zin

AU - Shein, Thinzer

AU - Han, Soe Soe

AU - Zaw, Ni Ni

AU - Han, Jin-Hee

AU - Lee, Seong-Kyun

AU - Muh, Fauzi

AU - Kim, Jung-Yeon

AU - Cho, Shin-Hyeong

AU - Lee, Sang-Eun

AU - Yang, Eun-Jeong

AU - Chang, Chulhun L.

AU - Han, Eun-Taek

PY - 2016/6/30

Y1 - 2016/6/30

N2 - In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.

AB - In the era of (pre) elimination setting, the prevalence of malaria has been decreasing in most of the previously endemic areas. Therefore, effective cost-and time-saving validated pooling strategy is needed for detection of malaria in low transmission settings. In this study, optimal pooling numbers and lowest detection limit were assessed using known density samples prepared systematically, followed by genomic DNA extraction and nested PCR. Pooling strategy that composed of 10 samples in 1 pool, 20 μl in 1 sample, was optimal, and the parasite density as low as 2 p/μl for both falciparum and vivax infection was enough for detection of malaria. This pooling method showed effectiveness for handling of a huge number of samples in low transmission settings.

KW - Plasmodium falciparum

KW - Plasmodium vivax

KW - Malaria

KW - Pooling strategy

KW - Low-transmission setting

KW - Nested PCR

U2 - 10.3347/kjp.2016.54.3.253

DO - 10.3347/kjp.2016.54.3.253

M3 - Article

SN - 0023-4001

VL - 54

SP - 253

EP - 259

JO - Korean Journal of Parasitology

JF - Korean Journal of Parasitology

IS - 3

ER -

Effective high-throughput blood pooling strategy before DNA extraction for detection of malaria in low-transmission settings

Abstract

Keywords

UN SDGs

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