Dynamic interplay of the SUMO and ERK pathways in regulating Elk-1 transcriptional activity

S H Yang, E Jaffray, R T Hay, A D Sharrocks

Research output: Contribution to journalArticlepeer-review

210 Citations (Scopus)

Abstract

The ETS domain transcription factor Elk-1 is a direct target of the MAP kinase pathways. Phosphorylation of the Elk-1 transcriptional activation domain by MAP kinases triggers its activation. However, Elk-1 also contains two domains with repressive activities. One of these, the R motif, appears to function by suppressing the activity of the activation domain. Here, we demonstrate that SUMO modification of the R motif is required for this repressive activity. A dynamic interplay exists between the activating ERK MAP kinase pathway and the repressive SUMO pathway. ERK pathway activation leads to both phosphorylation of Elk-1 and loss of SUMO conjugation and, hence, to the loss of the repressive activity of the R motif. Thus, the reciprocal regulation of the activation and repressive activities are coupled by MAP kinase modification of Elk-1.

Original languageEnglish
Pages (from-to)63-74
Number of pages12
JournalMolecular Cell
Volume12
Publication statusPublished - Jul 2003

Keywords

  • E3 LIGASE
  • HISTONE DEACETYLASE-1
  • COVALENT ATTACHMENT
  • REPRESSION DOMAIN
  • INHIBITORY DOMAIN
  • NUCLEAR-BODIES
  • PROTEIN
  • ACTIVATION
  • COMPLEX
  • CONTAINS

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