Duplication of the ribosomal gene cluster in the marine polychaete Platynereis dumerilii correlates with ITS polymorphism.

J. Hui, N. Kortchagina, D. Arendt, G. Balavoine, David Ellard Keith Ferrier

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Platynereis dumerilii is an emerging model system for both comparative developmental biology and ecotoxicology. As such it would be extremely valuable to be able to map the location of genes and markers in its genome, in order to understand how the genome changes or has changed, either due to chemical and environmental insults or during evolution. Here we report the adaptation of the technique of chromosomal fluorescence in si. tu hybridization (FISH) to P. dumerlii for the localization of single-copy genes. Also the duplicated ribosomal gene cluster of this annelid is more reliably revealed with FISH than with the previous technique of silver staining. This duplicated ribosomal cluster correlates with polymorphisms in the internal transcribed spacer (ITS) regions, which has implications for the use of these regions in phylogenetic or population biology work.

Original languageEnglish
Pages (from-to)443-449
Number of pages7
JournalJournal of the Marine Biological Association of the United Kingdom
Volume87
DOIs
Publication statusPublished - Apr 2007

Keywords

  • INTERNAL TRANSCRIBED SPACERS
  • CONCERTED EVOLUTION
  • DROSOPHILA-MELANOGASTER
  • DNA
  • CHROMOSOMES
  • SEQUENCES
  • CHAETOGNATHS
  • VERTEBRATES
  • PHYLOGENY
  • EXCHANGES

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