Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

Thomas J. Ashton; Meriem Kayoueche-Reeve; Andrew J. Blight; Jon Moore; David M. Paterson

doi:10.1017/S0025315416000813

Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

Thomas J. Ashton, Meriem Kayoueche-Reeve, Andrew J. Blight, Jon Moore, David M. Paterson

Research output: Contribution to journal › Article › peer-review

Abstract

Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

Original language	English
Pages (from-to)	1479-1482
Number of pages	4
Journal	Journal of the Marine Biological Association of the United Kingdom
Volume	97
Issue number	7
Early online date	9 Jun 2016
DOIs	https://doi.org/10.1017/S0025315416000813
Publication status	Published - Nov 2017

Keywords

Patella
Limpets
DNA barcoding
Mucus
Species determination

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10.1017/S0025315416000813

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@article{1240d9116a6a4e1486704813964709fb,

title = "Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling",

abstract = "Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.",

keywords = "Patella, Limpets, DNA barcoding, Mucus, Species determination",

author = "Ashton, {Thomas J.} and Meriem Kayoueche-Reeve and Blight, {Andrew J.} and Jon Moore and Paterson, {David M.}",

note = "This study was funded by the The Sullom Voe Association/Shetland Oil Terminal Environmental Advisory Group (SOTEAG) and David M. Paterson received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.",

year = "2017",

month = nov,

doi = "10.1017/S0025315416000813",

language = "English",

volume = "97",

pages = "1479--1482",

journal = "Journal of the Marine Biological Association of the United Kingdom",

issn = "0025-3154",

publisher = "Cambridge University Press",

number = "7",

}

Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling. / Ashton, Thomas J.; Kayoueche-Reeve, Meriem; Blight, Andrew J. et al.
In: Journal of the Marine Biological Association of the United Kingdom, Vol. 97, No. 7, 11.2017, p. 1479-1482.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

AU - Ashton, Thomas J.

AU - Kayoueche-Reeve, Meriem

AU - Blight, Andrew J.

AU - Moore, Jon

AU - Paterson, David M.

N1 - This study was funded by the The Sullom Voe Association/Shetland Oil Terminal Environmental Advisory Group (SOTEAG) and David M. Paterson received funding from the MASTS pooling initiative (The Marine Alliance for Science and Technology for Scotland) and their support is gratefully acknowledged. MASTS is funded by the Scottish Funding Council (grant reference HR09011) and contributing institutions.

PY - 2017/11

Y1 - 2017/11

N2 - Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

AB - Accurate discrimination of two morphologically similar species of Patella limpets has been facilitated by using qPCR amplification of species-specific mitochondrial genomic regions. Cost-effective and non-destructive sampling is achieved using a mucus swab and simple sample lysis and dilution to create a PCR template. Results show 100% concurrence with dissection and microscopic analysis, and the technique has been employed successfully in field studies. The use of highly sensitive DNA barcoding techniques such as this hold great potential for improving previously challenging field assessments of species abundance.

KW - Patella

KW - Limpets

KW - DNA barcoding

KW - Mucus

KW - Species determination

U2 - 10.1017/S0025315416000813

DO - 10.1017/S0025315416000813

M3 - Article

SN - 0025-3154

VL - 97

SP - 1479

EP - 1482

JO - Journal of the Marine Biological Association of the United Kingdom

JF - Journal of the Marine Biological Association of the United Kingdom

IS - 7

ER -

Duplex DNA barcoding allows accurate species determination of morphologically similar limpets (Patella spp.) from non-destructive sampling

Abstract

Keywords

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