Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample

Joanna E. Rivas Ramos; Paul R. Johnston; Robert J. H. Hammond; Matthew T. G. Holden; Derek J. Sloan; Andreas F. Haag

doi:10.1099/acmi.0.001110.v3

Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample

Joanna E. Rivas Ramos, Paul R. Johnston, Robert J. H. Hammond, Matthew T. G. Holden, Derek J. Sloan, Andreas F. Haag^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we report the draft sequence of a rapid-growing nontuberculous Mycobacterium isolated from a urine sample at the Scottish Mycobacteria Reference Laboratory, Royal Infirmary of Edinburgh, UK. The reported genome has a length of 6,749,454 bp, a G+C content of 67.2 mol% and 6,336 protein CDSs. Average nucleotide identity (ANI) analysis identified Mycobacterium vanbaalenii PYR-1 as the closest relative (83.32% ANI), indicating that this isolate likely represents a novel species within the genus. Notably, phenotypic characterization revealed a distinct antimicrobial resistance (AMR) profile. This assembly provides a valuable resource for studying the evolution of AMR mechanisms in nontuberculous mycobacteria and offers insight into resistance phenotypes observed in clinical isolates.

Original language	English
Article number	001110.v3
Pages (from-to)	1-4
Number of pages	4
Journal	Access Microbiology
Volume	8
Issue number	1
DOIs	https://doi.org/10.1099/acmi.0.001110.v3
Publication status	Published - 28 Jan 2026

Keywords

Antimicrobial susceptibility
Draft genome sequence
Mycobacterium
Non-tuberculous mycobacteria (NTM)
Phylogenetic analysis

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10.1099/acmi.0.001110.v3Licence: CC BY

RivasRamos-et-al-2026-Draft-genome-sequence-ACMI-001110.v3-CCBY
Copyright © 2026 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Final published version, 580 KBLicence: CC BY

Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample (dataset0
Johnston, P. (Creator), Hammond, R. (Creator), Sloan, D. (Creator) & Haag, A. (Creator), NCBI GenBank, 2025
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1229527/
Dataset

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title = "Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample",

abstract = "Here, we report the draft sequence of a rapid-growing nontuberculous Mycobacterium isolated from a urine sample at the Scottish Mycobacteria Reference Laboratory, Royal Infirmary of Edinburgh, UK. The reported genome has a length of 6,749,454 bp, a G+C content of 67.2 mol\% and 6,336 protein CDSs. Average nucleotide identity (ANI) analysis identified Mycobacterium vanbaalenii PYR-1 as the closest relative (83.32\% ANI), indicating that this isolate likely represents a novel species within the genus. Notably, phenotypic characterization revealed a distinct antimicrobial resistance (AMR) profile. This assembly provides a valuable resource for studying the evolution of AMR mechanisms in nontuberculous mycobacteria and offers insight into resistance phenotypes observed in clinical isolates.",

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AU - Johnston, Paul R.

AU - Hammond, Robert J. H.

AU - Holden, Matthew T. G.

AU - Sloan, Derek J.

AU - Haag, Andreas F.

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AB - Here, we report the draft sequence of a rapid-growing nontuberculous Mycobacterium isolated from a urine sample at the Scottish Mycobacteria Reference Laboratory, Royal Infirmary of Edinburgh, UK. The reported genome has a length of 6,749,454 bp, a G+C content of 67.2 mol% and 6,336 protein CDSs. Average nucleotide identity (ANI) analysis identified Mycobacterium vanbaalenii PYR-1 as the closest relative (83.32% ANI), indicating that this isolate likely represents a novel species within the genus. Notably, phenotypic characterization revealed a distinct antimicrobial resistance (AMR) profile. This assembly provides a valuable resource for studying the evolution of AMR mechanisms in nontuberculous mycobacteria and offers insight into resistance phenotypes observed in clinical isolates.

KW - Antimicrobial susceptibility

KW - Draft genome sequence

KW - Mycobacterium

KW - Non-tuberculous mycobacteria (NTM)

KW - Phylogenetic analysis

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Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample

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Keywords

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Datasets

Draft genome sequence of a non-tuberculous Mycobacterium strain isolated from a clinical urine sample (dataset0

Cite this