Does AMP-activated protein kinase couple inhibition of mitochondrial oxidative phosphorylation by hypoxia to calcium signaling in O2-sensing cells?

Anthony Mark Evans, KJW Mustard, CN Wyatt, C Peers, M Dipp, P Kumar, NP Kinnear, DG Hardie

Research output: Contribution to journalArticlepeer-review

158 Citations (Scopus)

Abstract

Specialized O-2-sensing cells exhibit a particularly low threshold to regulation by O-2 supply and function to maintain arterial pO(2) within physiological limits. For example, hypoxic pulmonary vasoconstriction optimizes ventilation-perfusion matching in the lung, whereas carotid body excitation elicits corrective cardio-respiratory reflexes. It is generally accepted that relatively mild hypoxia inhibits mitochondrial oxidative phosphorylation in O-2-sensing cells, thereby mediating, in part, cell activation. However, the mechanism by which this process couples to Ca2+ signaling mechanisms remains elusive, and investigation of previous hypotheses has generated contrary data and failed to unite the field. We propose that a rise in the cellular AMP/ATP ratio activates AMP-activated protein kinase and thereby evokes Ca2+ signals in O-2-sensing cells. Co-immunoprecipitation identified three possible AMP-activated protein kinase subunit isoform combinations in pulmonary arterial myocytes, with alpha 1 beta 2 gamma 1 predominant. Furthermore, their tissue-specific distribution suggested that the AMP-activated protein kinase-alpha 1 catalytic isoform may contribute, via amplification of the metabolic signal, to the pulmonary selectivity required for hypoxic pulmonary vasoconstriction. Immunocytochemistry showed AMP-activated protein kinase-alpha 1 to be located throughout the cytoplasm of pulmonary arterial myocytes. In contrast, it was targeted to the plasma membrane in carotid body glomus cells. Consistent with these observations and the effects of hypoxia, stimulation of AMP-activated protein kinase by phenformin or 5-aminoimidazole-4-carboxamide- riboside elicited discrete Ca2+ signaling mechanisms in each cell type, namely cyclic ADP-ribose-dependent Ca2+ mobilization from the sarcoplasmic reticulum via ryanodine receptors in pulmonary arterial myocytes and transmembrane Ca2+ influx into carotid body glomus cells. Thus, metabolic sensing by AMP-activated protein kinase may mediate chemotransduction by hypoxia.

Original languageEnglish
Pages (from-to)41504-41511
Number of pages8
JournalJournal of Biological Chemistry
Volume280
Issue number50
DOIs
Publication statusPublished - 16 Dec 2005

Keywords

  • CYCLIC ADP-RIBOSE
  • ARTERY SMOOTH-MUSCLE
  • BODY GLOMUS CELLS
  • CAROTID-BODY
  • PULMONARY VASOCONSTRICTION
  • RESPIRATORY-CHAIN
  • I CELLS
  • CHEMORECEPTOR CELLS
  • UPSTREAM KINASE
  • K+ CURRENTS

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