DNA analysis of breast tumour fine needle aspirates using flow cytometry

P A Levack; P Mullen; T J Anderson; W R Miller; A P Forrest

doi:10.1038/bjc.1987.259

DNA analysis of breast tumour fine needle aspirates using flow cytometry

P A Levack, P Mullen, T J Anderson, W R Miller, A P Forrest

Research output: Contribution to journal › Article › peer-review

Abstract

Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy.

Original language	English
Pages (from-to)	643-6
Number of pages	4
Journal	British Journal of Cancer
Volume	56
Issue number	5
DOIs	https://doi.org/10.1038/bjc.1987.259
Publication status	Published - Nov 1987

Keywords

Adult
Aged
Biopsy, Needle
Breast Neoplasms/analysis
DNA, Neoplasm/analysis
Female
Flow Cytometry
Humans
Middle Aged
Ploidies

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/bjc.1987.259

Cite this

@article{a68dc96ff2fb401781eecdb416bb2174,

title = "DNA analysis of breast tumour fine needle aspirates using flow cytometry",

abstract = "Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy.",

keywords = "Adult, Aged, Biopsy, Needle, Breast Neoplasms/analysis, DNA, Neoplasm/analysis, Female, Flow Cytometry, Humans, Middle Aged, Ploidies",

author = "Levack, {P A} and P Mullen and Anderson, {T J} and Miller, {W R} and Forrest, {A P}",

year = "1987",

month = nov,

doi = "10.1038/bjc.1987.259",

language = "English",

volume = "56",

pages = "643--6",

journal = "British Journal of Cancer",

issn = "0007-0920",

publisher = "Nature publishing group",

number = "5",

}

TY - JOUR

T1 - DNA analysis of breast tumour fine needle aspirates using flow cytometry

AU - Levack, P A

AU - Mullen, P

AU - Anderson, T J

AU - Miller, W R

AU - Forrest, A P

PY - 1987/11

Y1 - 1987/11

N2 - Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy.

AB - Cellular DNA was analysed by flow cytometry in fine needles aspirates (FNA) from both benign and malignant breast lesions in order to determine the feasibility of flow cytometric analysis. In 22 of 26 (84%) benign and 69 of 74 (93%) malignant aspirates, sufficient cells were present to produce good quality DNA histograms. DNA in all 22 benign lesions was diploid. In contrast, of the 69 cancers with sufficient cells for analysis, 40.6% had a diploid DNA content alone, whilst 59.4% had an additional DNA aneuploid line. These results indicate that the majority of FNAs provide sufficient material for flow cytometric analysis of DNA profiles. Such aspirates taken in a sequential manner may also prove to be an ideal method of studying tumour response to therapy.

KW - Adult

KW - Aged

KW - Biopsy, Needle

KW - Breast Neoplasms/analysis

KW - DNA, Neoplasm/analysis

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Middle Aged

KW - Ploidies

U2 - 10.1038/bjc.1987.259

DO - 10.1038/bjc.1987.259

M3 - Article

C2 - 3426930

SN - 0007-0920

VL - 56

SP - 643

EP - 646

JO - British Journal of Cancer

JF - British Journal of Cancer

IS - 5

ER -

DNA analysis of breast tumour fine needle aspirates using flow cytometry

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this