Distinct intracellular calcium transients in neurites and somata integrate neuronal signals

FW Johenning, M Zochowski, Stuart John Conway, AB Holmes, P Koulen, BE Ehrlich

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

Intracellular calcium signals have distinct temporal and spatial patterns in neurons in which signal initiation and repetitive spiking occurs predominantly in the neurite. We investigated the functional implications of the coexpression of different isoforms of ryanodine receptors (RyR) and inositol 1,4,5-trisphosphate receptors (InsP3Rs) using immunocytochemistry, Western blotting, and calcium imaging in neuronally differentiated PC12 cells. InsP3R type III, an isoform that has been shown to be upregulated in neuronal apoptosis, is exclusively expressed in the soma, serving as a gatekeeper for high-magnitude calcium surges. InsP3R type I is expressed throughout the cell and can be related to signal initiation and repetitive spiking in the neurite. RyR types 2 and 3 are distributed throughout the cell. In the soma, they serve as amplifying molecular switches, facilitating recruitment of the InsP3R type III-dependent pool. In the neurite, they decrease the probability of repetitive spiking. Use of a cell-permeant analog of InsP3 suggested that regional specificity in InsP3 production and surface-to-volume effects play minor roles in determining temporal and spatial calcium signaling patterns in neurons. Our findings suggest that additional modulatory processes acting on the intracellular channels are necessary to generate spatially specific calcium signaling.

Original languageEnglish
Pages (from-to)5344-5353
Number of pages10
JournalThe Journal of Neuroscience
Volume22
Publication statusPublished - 1 Jul 2002

Keywords

  • intracellular calcium signaling
  • inositol 1,4,5 trisphosphate
  • InsP3 receptor
  • ryanodine receptor
  • PC12 cells
  • neurite
  • soma
  • INOSITOL 1,4,5-TRISPHOSPHATE RECEPTORS
  • BACKPROPAGATING ACTION-POTENTIALS
  • III INSP(3) RECEPTOR
  • NERVE GROWTH-FACTOR
  • CA2+ RELEASE
  • PC12 CELLS
  • RYANODINE RECEPTORS
  • ENDOPLASMIC-RETICULUM
  • PYRAMIDAL NEURONS
  • CHANNELS

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