Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

Li Hao; Matthew T. G. Holden; Xin Wang; Lubomira Andrew; Sabine Wellnitz; Fang Hu; Melissa Whaley; Scott Sammons; Kristen Knipe; Mike Frace; Lucy A McNamara; Paul Liberator; Annaliesa S Anderson

doi:10.1099/mgen.0.000155

Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

Li Hao, Matthew T. G. Holden, Xin Wang, Lubomira Andrew, Sabine Wellnitz, Fang Hu, Melissa Whaley, Scott Sammons, Kristen Knipe, Mike Frace, Lucy A McNamara, Paul Liberator, Annaliesa S Anderson

Research output: Contribution to journal › Article › peer-review

Abstract

Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first at University A in New Jersey included nine confirmed cases reported between March 2013 and March 2014. The second outbreak occurred at University B in California, with four confirmed cases during November 2013. The public health response to these outbreaks included the approval and deployment of a serogroup B meningococcal vaccine that was not yet licensed in the USA. This study investigated the use of whole-genome sequencing(WGS) to examine the genetic profile of the disease-causing outbreak isolates at each university. Comparative WGS revealed differences in evolutionary patterns between the two disease outbreaks. The University A outbreak isolates were very closely related, with differences primarily attributed to single nucleotide polymorphisms/insertion-deletion (SNP/indel) events. In contrast, the University B outbreak isolates segregated into two phylogenetic clades, differing in large part due to recombination events covering extensive regions (>30 kb) of the genome including virulence factors. This high-resolution comparison of two meningococcal disease outbreaks further demonstrates the genetic complexity of meningococcal bacteria as related to evolution and disease virulence.

Original language	English
Article number	e000155
Number of pages	10
Journal	Microbial Genomics
Volume	4
Issue number	4
Early online date	4 Apr 2018
DOIs	https://doi.org/10.1099/mgen.0.000155
Publication status	Published - Apr 2018

Keywords

Serogroup B Neisseria meningitidis
Disease outbreak
Multi-locus sequence typing (MLST)
Whole genome sequence (WGS)
Single nucleotide polymorphism (SNP)
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1099/mgen.0.000155Licence: CC BY

Holden_2018_Evolutionarypatterns_CC
© 2018 The Authors. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Final published version, 911 KBLicence: CC BY

Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA (dataset)
Hao, L. (Creator), Holden, M. T. G. (Creator), Wang, X. (Creator), Andrew, L. (Creator), Wellnitz, S. (Creator), Hu, F. (Creator), Whaley, M. (Creator), Sammons, S. (Creator), Knipe, K. (Creator), Frace, M. (Creator), McNamara, L. A. (Creator), Liberator, P. (Creator) & Anderson, A. S. (Creator), National Center for Biotechnology Information, 21 Jul 2017
https://www.ncbi.nlm.nih.gov/bioproject/395340
Dataset

Cite this

Hao, L., Holden, M. T. G., Wang, X., Andrew, L., Wellnitz, S., Hu, F., Whaley, M., Sammons, S., Knipe, K., Frace, M., McNamara, L. A., Liberator, P., & Anderson, A. S. (2018). Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA. Microbial Genomics, 4(4), Article e000155. https://doi.org/10.1099/mgen.0.000155

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title = "Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA",

abstract = "Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first at University A in New Jersey included nine confirmed cases reported between March 2013 and March 2014. The second outbreak occurred at University B in California, with four confirmed cases during November 2013. The public health response to these outbreaks included the approval and deployment of a serogroup B meningococcal vaccine that was not yet licensed in the USA. This study investigated the use of whole-genome sequencing(WGS) to examine the genetic profile of the disease-causing outbreak isolates at each university. Comparative WGS revealed differences in evolutionary patterns between the two disease outbreaks. The University A outbreak isolates were very closely related, with differences primarily attributed to single nucleotide polymorphisms/insertion-deletion (SNP/indel) events. In contrast, the University B outbreak isolates segregated into two phylogenetic clades, differing in large part due to recombination events covering extensive regions (>30 kb) of the genome including virulence factors. This high-resolution comparison of two meningococcal disease outbreaks further demonstrates the genetic complexity of meningococcal bacteria as related to evolution and disease virulence.",

keywords = "Serogroup B Neisseria meningitidis, Disease outbreak, Multi-locus sequence typing (MLST), Whole genome sequence (WGS), Single nucleotide polymorphism (SNP), Vaccine",

author = "Li Hao and Holden, {Matthew T. G.} and Xin Wang and Lubomira Andrew and Sabine Wellnitz and Fang Hu and Melissa Whaley and Scott Sammons and Kristen Knipe and Mike Frace and McNamara, {Lucy A} and Paul Liberator and Anderson, {Annaliesa S}",

note = "L. H., L. A., S. W., P. L. and A. S. A. are current employees of Pfizer, and this work was funded by Pfizer Inc.",

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doi = "10.1099/mgen.0.000155",

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AU - Hao, Li

AU - Holden, Matthew T. G.

AU - Wang, Xin

AU - Andrew, Lubomira

AU - Wellnitz, Sabine

AU - Hu, Fang

AU - Whaley, Melissa

AU - Sammons, Scott

AU - Knipe, Kristen

AU - Frace, Mike

AU - McNamara, Lucy A

AU - Liberator, Paul

AU - Anderson, Annaliesa S

N1 - L. H., L. A., S. W., P. L. and A. S. A. are current employees of Pfizer, and this work was funded by Pfizer Inc.

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N2 - Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first at University A in New Jersey included nine confirmed cases reported between March 2013 and March 2014. The second outbreak occurred at University B in California, with four confirmed cases during November 2013. The public health response to these outbreaks included the approval and deployment of a serogroup B meningococcal vaccine that was not yet licensed in the USA. This study investigated the use of whole-genome sequencing(WGS) to examine the genetic profile of the disease-causing outbreak isolates at each university. Comparative WGS revealed differences in evolutionary patterns between the two disease outbreaks. The University A outbreak isolates were very closely related, with differences primarily attributed to single nucleotide polymorphisms/insertion-deletion (SNP/indel) events. In contrast, the University B outbreak isolates segregated into two phylogenetic clades, differing in large part due to recombination events covering extensive regions (>30 kb) of the genome including virulence factors. This high-resolution comparison of two meningococcal disease outbreaks further demonstrates the genetic complexity of meningococcal bacteria as related to evolution and disease virulence.

AB - Neisseria meningitidis serogroup B (MnB) was responsible for two independent meningococcal disease outbreaks at universities in the USA during 2013. The first at University A in New Jersey included nine confirmed cases reported between March 2013 and March 2014. The second outbreak occurred at University B in California, with four confirmed cases during November 2013. The public health response to these outbreaks included the approval and deployment of a serogroup B meningococcal vaccine that was not yet licensed in the USA. This study investigated the use of whole-genome sequencing(WGS) to examine the genetic profile of the disease-causing outbreak isolates at each university. Comparative WGS revealed differences in evolutionary patterns between the two disease outbreaks. The University A outbreak isolates were very closely related, with differences primarily attributed to single nucleotide polymorphisms/insertion-deletion (SNP/indel) events. In contrast, the University B outbreak isolates segregated into two phylogenetic clades, differing in large part due to recombination events covering extensive regions (>30 kb) of the genome including virulence factors. This high-resolution comparison of two meningococcal disease outbreaks further demonstrates the genetic complexity of meningococcal bacteria as related to evolution and disease virulence.

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KW - Disease outbreak

KW - Multi-locus sequence typing (MLST)

KW - Whole genome sequence (WGS)

KW - Single nucleotide polymorphism (SNP)

KW - Vaccine

U2 - 10.1099/mgen.0.000155

DO - 10.1099/mgen.0.000155

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JO - Microbial Genomics

JF - Microbial Genomics

IS - 4

M1 - e000155

ER -

Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA

Abstract

Keywords

UN SDGs

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Datasets

Distinct evolutionary patterns of Neisseria meningitidis serogroup B disease outbreaks at two universities in the USA (dataset)

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