Distance measurements in the borderline region of applicability of CW EPR and DEER: A model study on a homologous series of spin-labelled peptides

Janet Eleanor Banham, C. M. Baker, S. Ceola, I. J. Day, G. H. Grant, E. J. J. Groenen, C. T. Rodgers, G. Jeschke, C. R. Timmel*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)

Abstract

Inter-spin distances between 1 nm and 4.5 nm are measured by continuous wave (CW) and pulsed electron paramagnetic resonance (EPR) methods for a series of nitroxide-spin-labelled peptides. The upper distance limit for measuring dipolar coupling by the broadening of the CW spectrum and the lower distance limit for the present optimally-adjusted double electron electron resonance (DEER) set-up are determined and found to be both around 1.6-1.9 nm. The methods for determining distances and corresponding distributions from CW spectral line broadening are reviewed and further developed. Also, the work shows that a correction factor is required for the analysis of inter-spin distances below approximately 2 nm for DEER measurements and this is calculated using the density matrix formalism. (c) 2007 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)202-218
Number of pages17
JournalJournal of Magnetic Resonance
Volume191
Issue number2
DOIs
Publication statusPublished - Apr 2008

Keywords

  • DEER
  • EPR spectroscopy
  • structure elucidation
  • dipolar interactions
  • spin labels
  • COMPREHENSIVE SOFTWARE PACKAGE
  • PULSE EPR
  • SPECTROSCOPY
  • PROTEINS
  • DYNAMICS
  • PELDOR

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