Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor

A Straight, A Cheung, J Limouze, I Chen, Nicholas James Westwood, J R Sellers, T J Mitchison

Research output: Contribution to journalArticlepeer-review

1057 Citations (Scopus)

Abstract

Completion of cell division during cytokinesis requires temporally and spatially regulated communication from the microtubule cytoskeleton to the actin cytoskeleton and the cell membrane. We identified a specific inhibitor of nonmuscle myosin II, blebbistatin, that inhibited contraction of the cleavage furrow without disrupting mitosis or contractile ring assembly. Using blebbistatin and other drugs, we showed that exit from the cytokinetic phase of the cell cycle depends on ubiquitin-mediated proteolysis. Continuous signals from microtubules are required to maintain the position of the cleavage furrow, and these signals control the localization of myosin II independently of other furrow components.

Original languageEnglish
Pages (from-to)1743-1747
Number of pages5
JournalScience
Volume299
Issue number5613
DOIs
Publication statusPublished - 14 Mar 2003

Keywords

  • CONTRACTILE RING
  • PROTEIN-KINASE
  • MAMMALIAN-CELLS
  • CLEAVAGE FURROW
  • MITOTIC SPINDLE
  • MICROTUBULES
  • LOCALIZATION
  • PROTEOLYSIS
  • ANAPHASE
  • ANTIBODY

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